Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists

The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental...

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Detalles Bibliográficos
Autores principales:	Vittone, Leticia Beatriz, Mundiña-Weilenmann, Cecilia, Chiappe de Cingolani, Gladys Ethel, Mattiazzi, Alicia Ramona
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	1993
Materias:	Ciencias Médicas myocardial relaxation phospholamban Ca2+-calmodulin dependent phosphorylation W7
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/140836
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-140836
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas myocardial relaxation phospholamban Ca2+-calmodulin dependent phosphorylation W7
spellingShingle	Ciencias Médicas myocardial relaxation phospholamban Ca2+-calmodulin dependent phosphorylation W7 Vittone, Leticia Beatriz Mundiña-Weilenmann, Cecilia Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
topic_facet	Ciencias Médicas myocardial relaxation phospholamban Ca2+-calmodulin dependent phosphorylation W7
description	The role of the Ca2+-calmodulin dependent pathway of phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists was studied in isolated perfused rat heart. Administration of the calmodulin antagonist W7 or lowering [Ca]0 from 1.35 mM (control) to 0.25 mM, were used as experimental tools to inhibit the Ca2+-calmodulin dependent protein kinase activity. 3×10−8 M isoproterenol increased cAMP levels from 0.613±0.109 pmol/mg wet weight to 1.581±0.123, phospholamban phosphorylation from 36±6 pmol32P/mg protein to 277±26 and decreased time to half relaxation (t1/2) from 61±2 msec to 39±2. Simultaneous perfusion of isoproterenol with 10−6 M W7, decreased phospholamban phosphorylation to 170±23 and prolongated t1/2 to 47±3 but did not affect the increase either in cAMP levels or myocardial contractility produced by isoproterenol. Similar effects on phospholamban phosphorylation and myocardial relaxation were obtained when isoproterenol was perfused in low [Ca]0. Low [Ca]0 did not affect the increase in cAMP elicited by isoproterenol but offset the positive inotropic effect of the β-agonist.
format	Articulo Articulo
author	Vittone, Leticia Beatriz Mundiña-Weilenmann, Cecilia Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona
author_facet	Vittone, Leticia Beatriz Mundiña-Weilenmann, Cecilia Chiappe de Cingolani, Gladys Ethel Mattiazzi, Alicia Ramona
author_sort	Vittone, Leticia Beatriz
title	Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_short	Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_full	Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_fullStr	Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_full_unstemmed	Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
title_sort	role of ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists
publishDate	1993
url	http://sedici.unlp.edu.ar/handle/10915/140836
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Role of Ca2+-calmodulin dependent phospholamban phosphorylation on the relaxant effect of β-adrenergic agonists

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