Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize

Zea mays ssp. mays cv. Colorado Klein organogenic calli were initiated from 11 daysold embryos on basic medium containing 2.25 μM 2, 4-D. The callus originated from one of the embryos was subcultured every month to fresh medium supplemented with 4.5 or 9 μM 2, 4-D, for 84months. The first chromosoma...

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Autores principales: Molina, María del Carmen, García, Maria Dina
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Ciencias Agrarias
Somaclonal variation
Long-term callus cultures
Chromosomal abnormalities
2,4-D
Maize regeneration
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139405
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139405
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Agrarias
Somaclonal variation
Long-term callus cultures
Chromosomal abnormalities
2,4-D
Maize regeneration
spellingShingle Ciencias Agrarias
Somaclonal variation
Long-term callus cultures
Chromosomal abnormalities
2,4-D
Maize regeneration
Molina, María del Carmen
García, Maria Dina
Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
topic_facet Ciencias Agrarias
Somaclonal variation
Long-term callus cultures
Chromosomal abnormalities
2,4-D
Maize regeneration
description Zea mays ssp. mays cv. Colorado Klein organogenic calli were initiated from 11 daysold embryos on basic medium containing 2.25 μM 2, 4-D. The callus originated from one of the embryos was subcultured every month to fresh medium supplemented with 4.5 or 9 μM 2, 4-D, for 84months. The first chromosomal abnormalities appeared after 17 months of culture : 70% of regenerated plants were normal, 20% aneuploid and the remaining 10% were tetraploid. Thereafter chromosomal aberrations increased. Most (95%) of the plants regenerated after 32 months had one extra chromosome, and meiotic analysis of regenerants showed deficiencies, duplications, inversions, translocations and ring chromosomes. Chromosome number of cells from 60 months old callus ranged from 2n=18 to 2n=23, but 90% of regenerated plants had 2n=21 and the remaining 10% had 2n=20. Plants regenerated from 64 to 84 months old callus were sterile. Also, phenotypic alterations were observed in plants regenerated from 32 months and older calli. Although original maize plants are yellow flint, 75% of R0 ears showed white flint kernels and their progeny exhibited yellow flint, white flint, white dent and white sugary caryopses.
format Articulo
Articulo
author Molina, María del Carmen
García, Maria Dina
author_facet Molina, María del Carmen
García, Maria Dina
author_sort Molina, María del Carmen
title Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
title_short Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
title_full Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
title_fullStr Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
title_full_unstemmed Analysis of Genetic Variability in Long-term Callus Cultures and Regenerated Plants of Maize
title_sort analysis of genetic variability in long-term callus cultures and regenerated plants of maize
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/139405
work_keys_str_mv AT molinamariadelcarmen analysisofgeneticvariabilityinlongtermcallusculturesandregeneratedplantsofmaize
AT garciamariadina analysisofgeneticvariabilityinlongtermcallusculturesandregeneratedplantsofmaize
bdutipo_str Repositorios
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