Antioxidant Profiling of Ginger via Reaction Flow Chromatography

Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective...

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Detalles Bibliográficos
Autores principales: Zhou, Xian, Power, Declan, Jones, Andy, Acquaviva, Agustín, Dennis, Gary R., Shalliker, R. Andrew, Li, Chun Guang, Soliven, Arianne
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Química
Antioxidant
Reaction flow chromatography
Ferric reducing antioxidant power assay
Ginger
Post column derivatization
Selective detection
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139204
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139204
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Antioxidant
Reaction flow chromatography
Ferric reducing antioxidant power assay
Ginger
Post column derivatization
Selective detection
spellingShingle Química
Antioxidant
Reaction flow chromatography
Ferric reducing antioxidant power assay
Ginger
Post column derivatization
Selective detection
Zhou, Xian
Power, Declan
Jones, Andy
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chun Guang
Soliven, Arianne
Antioxidant Profiling of Ginger via Reaction Flow Chromatography
topic_facet Química
Antioxidant
Reaction flow chromatography
Ferric reducing antioxidant power assay
Ginger
Post column derivatization
Selective detection
description Reaction flow (RF) chromatography is a powerful and efficient approach that utilizes conventional high-performance liquid chromatography (HPLC)–ultraviolet (UV)–visible detection. This technique exploits a novel column end-fitting and an extra HPLC pump that delivers a reagent specific for selective detection, in particular the antioxidant profiling of natural products. This study employed RF for the first time to identify antioxidants in a commercial ginger sample. This demonstrated the previously validated assay’s ease and power to extract information about the natural product’s antioxidant properties. Due to the simplicity involved with data analysis and peak matching process, the following information was revealed between the chemical and antioxidant profiles: three of the strongest antioxidant activity peaks in the ginger sample (593 nm) did not correlate with the three most abundant chemical profile peaks (UV absorbance at 254 and 280 nm); the ratio of seven antioxidant peaks may be potentially used for food authenticity purposes, and future research should target these peaks for the early discovery of novel antioxidants sourced in ginger. Utilization of this previously validated assay provided the resolution of numerous peaks in the ginger extract and information associated with their antioxidant attributes and chemical abundance. This approach is more informative than total antioxidant assays that lack compound specificity information. Furthermore, it is superior to mass spectrometric (MS) assays that cannot evaluate each compound’s antioxidant strength, and does not involve the expense involved in the acquisition and maintenance of the MS detection hardware, and does not require the high level of expertise needed to conduct the MS data analysis.
format Articulo
Articulo
author Zhou, Xian
Power, Declan
Jones, Andy
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chun Guang
Soliven, Arianne
author_facet Zhou, Xian
Power, Declan
Jones, Andy
Acquaviva, Agustín
Dennis, Gary R.
Shalliker, R. Andrew
Li, Chun Guang
Soliven, Arianne
author_sort Zhou, Xian
title Antioxidant Profiling of Ginger via Reaction Flow Chromatography
title_short Antioxidant Profiling of Ginger via Reaction Flow Chromatography
title_full Antioxidant Profiling of Ginger via Reaction Flow Chromatography
title_fullStr Antioxidant Profiling of Ginger via Reaction Flow Chromatography
title_full_unstemmed Antioxidant Profiling of Ginger via Reaction Flow Chromatography
title_sort antioxidant profiling of ginger via reaction flow chromatography
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/139204
work_keys_str_mv AT zhouxian antioxidantprofilingofgingerviareactionflowchromatography
AT powerdeclan antioxidantprofilingofgingerviareactionflowchromatography
AT jonesandy antioxidantprofilingofgingerviareactionflowchromatography
AT acquavivaagustin antioxidantprofilingofgingerviareactionflowchromatography
AT dennisgaryr antioxidantprofilingofgingerviareactionflowchromatography
AT shallikerrandrew antioxidantprofilingofgingerviareactionflowchromatography
AT lichunguang antioxidantprofilingofgingerviareactionflowchromatography
AT solivenarianne antioxidantprofilingofgingerviareactionflowchromatography
bdutipo_str Repositorios
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