Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study

The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assess...

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Detalles Bibliográficos
Autores principales: Vázquez, Diego E., Latorre Estivalis, José Manuel, Ons, Sheila, Farina, Walter M.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/139120
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-139120
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
spellingShingle Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
Vázquez, Diego E.
Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
topic_facet Ciencias Exactas
Biología
Apis mellifera
RNA-Seq
in vitro rearing
transcriptomic
energy metabolism
description The honey bee Apis mellifera is the most abundant managed pollinator in diverse crops worldwide. Consequently, it is exposed to a plethora of environmental stressors, among which are the agrochemicals. In agroecosystems, the herbicide glyphosate (GLY) is one of the most applied. In laboratory assessments, GLY affects the honey bee larval development by delaying its moulting, among other negative effects. However, it is still unknown how GLY affects larval physiology when there are no observable signs of toxicity. We carried out a longitudinal experimental design using the in vitro rearing procedure. Larvae were fed with food containing or not a sub-lethal dose of GLY in chronic exposure (120 h). Individuals without observable signs of toxicity were sampled and their gene expression profile was analyzed with a transcriptomic approach to compare between treatments. Even though 29% of larvae were asymptomatic in the exposed group, they showed transcriptional changes in several genes after the GLY chronic intake. A total of 19 transcripts were found to be differentially expressed in the RNA-Seq experiment, mainly linked with defensive response and intermediary metabolism processes. Furthermore, the enriched functional categories in the transcriptome of the exposed asymptomatic larvae were linked with enzymes with catalytic and redox activity. Our results suggest an enhanced catabolism and oxidative metabolism in honey bee larvae as a consequence of the sub-lethal exposure to GLY, even in the absence of observable symptoms.
format Articulo
Articulo
author Vázquez, Diego E.
Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
author_facet Vázquez, Diego E.
Latorre Estivalis, José Manuel
Ons, Sheila
Farina, Walter M.
author_sort Vázquez, Diego E.
title Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_short Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_full Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_fullStr Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_full_unstemmed Chronic exposure to glyphosate induces transcriptional changes in honey bee larva : A toxicogenomic study
title_sort chronic exposure to glyphosate induces transcriptional changes in honey bee larva : a toxicogenomic study
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/139120
work_keys_str_mv AT vazquezdiegoe chronicexposuretoglyphosateinducestranscriptionalchangesinhoneybeelarvaatoxicogenomicstudy
AT latorreestivalisjosemanuel chronicexposuretoglyphosateinducestranscriptionalchangesinhoneybeelarvaatoxicogenomicstudy
AT onssheila chronicexposuretoglyphosateinducestranscriptionalchangesinhoneybeelarvaatoxicogenomicstudy
AT farinawalterm chronicexposuretoglyphosateinducestranscriptionalchangesinhoneybeelarvaatoxicogenomicstudy
bdutipo_str Repositorios
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