Vacuolar Targeting and Characterization of Recombinant Antibodies

Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted t...

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Detalles Bibliográficos
Autores principales:	Ocampo, Carolina Gabriela, Petruccelli, Silvana, Pereira, Cláudia
Formato:	Libro Capitulo de libro
Lenguaje:	Inglés
Publicado:	Humana 2018
Materias:	Ciencias Exactas Biología Immunoglobulin N-glycosylation Vacuolar sorting signals Secretory pathway Vacuolar transport Molecular farming
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/138728
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-138728
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Biología Immunoglobulin N-glycosylation Vacuolar sorting signals Secretory pathway Vacuolar transport Molecular farming
spellingShingle	Ciencias Exactas Biología Immunoglobulin N-glycosylation Vacuolar sorting signals Secretory pathway Vacuolar transport Molecular farming Ocampo, Carolina Gabriela Petruccelli, Silvana Pereira, Cláudia Vacuolar Targeting and Characterization of Recombinant Antibodies
topic_facet	Ciencias Exactas Biología Immunoglobulin N-glycosylation Vacuolar sorting signals Secretory pathway Vacuolar transport Molecular farming
description	Plant-based platforms are extensively use for the expression of recombinant proteins, including monoclonal antibodies (mAbs). Generally, immunoglobulins (Igs) are sorted to the apoplast, which is often afflicted with intense proteolysis. Here, we describe methods to transiently express mAbs sorted to central vacuole in Nicotiana benthamiana leaves and to characterize the obtained IgG. Central vacuole is an appropriate compartment for the efficient production of Abs, consequently vacuolar sorting should be considered as an alternative strategy to obtain high protein yields.
format	Libro Capitulo de libro
author	Ocampo, Carolina Gabriela Petruccelli, Silvana Pereira, Cláudia
author_facet	Ocampo, Carolina Gabriela Petruccelli, Silvana Pereira, Cláudia
author_sort	Ocampo, Carolina Gabriela
title	Vacuolar Targeting and Characterization of Recombinant Antibodies
title_short	Vacuolar Targeting and Characterization of Recombinant Antibodies
title_full	Vacuolar Targeting and Characterization of Recombinant Antibodies
title_fullStr	Vacuolar Targeting and Characterization of Recombinant Antibodies
title_full_unstemmed	Vacuolar Targeting and Characterization of Recombinant Antibodies
title_sort	vacuolar targeting and characterization of recombinant antibodies
publisher	Humana
publishDate	2018
url	http://sedici.unlp.edu.ar/handle/10915/138728
work_keys_str_mv	AT ocampocarolinagabriela vacuolartargetingandcharacterizationofrecombinantantibodies AT petruccellisilvana vacuolartargetingandcharacterizationofrecombinantantibodies AT pereiraclaudia vacuolartargetingandcharacterizationofrecombinantantibodies
bdutipo_str	Repositorios
_version_	1764820457853288449

Vacuolar Targeting and Characterization of Recombinant Antibodies

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