Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization

Erythrocytes are widely used as a model system for membrane studies due to their relatively simple structure (they lack nuclei and organelles having only the plasma membrane), their convenient experimental manipulation and availability. However, the high hemoglobin content inside the red blood cells...

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Autores principales: Sánchez, Susana A., Herlax, Vanesa Silvana, Lillo, M. Pilar, Sandoval, Catalina, Aguilar, Joao, Günther, Germán
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2018
Materias:
Ciencias Médicas
Eritrocitos
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138562
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SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138562
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Eritrocitos
spellingShingle Ciencias Médicas
Eritrocitos
Sánchez, Susana A.
Herlax, Vanesa Silvana
Lillo, M. Pilar
Sandoval, Catalina
Aguilar, Joao
Günther, Germán
Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
topic_facet Ciencias Médicas
Eritrocitos
description Erythrocytes are widely used as a model system for membrane studies due to their relatively simple structure (they lack nuclei and organelles having only the plasma membrane), their convenient experimental manipulation and availability. However, the high hemoglobin content inside the red blood cells may be a problem for the use of fluorescent membrane probes. Hemoglobin content inside erythrocytes is around 20 mM and the molecules near the inner membrane surface would be the responsible for the quenching of fluorescent dyes such as 1,6-diphenyl-l,3,5-hexatriene (DPH) and 12-(9-anthroyl) stearic acid (AS).
format Articulo
Comunicacion
author Sánchez, Susana A.
Herlax, Vanesa Silvana
Lillo, M. Pilar
Sandoval, Catalina
Aguilar, Joao
Günther, Germán
author_facet Sánchez, Susana A.
Herlax, Vanesa Silvana
Lillo, M. Pilar
Sandoval, Catalina
Aguilar, Joao
Günther, Germán
author_sort Sánchez, Susana A.
title Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
title_short Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
title_full Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
title_fullStr Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
title_full_unstemmed Using Laurdan and Spectral Phasor Analysis to Study Erythrocytes Membrane Solubilization
title_sort using laurdan and spectral phasor analysis to study erythrocytes membrane solubilization
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/138562
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