Restriction site patterns in the ribosomal DNA of Camelidae

The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17...

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Autores principales: Semorile, Liliana Carmen, Crisci, Jorge Víctor, Arbeletche de Vidal Rioja, Lidia Beatriz
Formato: Articulo
Lenguaje:Inglés
Publicado: 1994
Materias:
Biología
camelids
ribosomal DNA
restriction map
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/138433
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-138433
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
camelids
ribosomal DNA
restriction map
spellingShingle Biología
camelids
ribosomal DNA
restriction map
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
Restriction site patterns in the ribosomal DNA of Camelidae
topic_facet Biología
camelids
ribosomal DNA
restriction map
description The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI, BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.
format Articulo
Articulo
author Semorile, Liliana Carmen
Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
author_facet Semorile, Liliana Carmen
Crisci, Jorge Víctor
Arbeletche de Vidal Rioja, Lidia Beatriz
author_sort Semorile, Liliana Carmen
title Restriction site patterns in the ribosomal DNA of Camelidae
title_short Restriction site patterns in the ribosomal DNA of Camelidae
title_full Restriction site patterns in the ribosomal DNA of Camelidae
title_fullStr Restriction site patterns in the ribosomal DNA of Camelidae
title_full_unstemmed Restriction site patterns in the ribosomal DNA of Camelidae
title_sort restriction site patterns in the ribosomal dna of camelidae
publishDate 1994
url http://sedici.unlp.edu.ar/handle/10915/138433
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AT criscijorgevictor restrictionsitepatternsintheribosomaldnaofcamelidae
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bdutipo_str Repositorios
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