Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae

We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues...

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Detalles Bibliográficos
Autores principales: Dikgolz, Vanesa Eva, Toledo, Andrea Vanesa, Topa, Pascual Emilio, López Lastra, Claudia Cristina
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Biología
Transverse Section
Entomopathogenic Fungus
Mosquito Larva
Fungal Structure
Histological Technique
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137558
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137558
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Transverse Section
Entomopathogenic Fungus
Mosquito Larva
Fungal Structure
Histological Technique
spellingShingle Biología
Transverse Section
Entomopathogenic Fungus
Mosquito Larva
Fungal Structure
Histological Technique
Dikgolz, Vanesa Eva
Toledo, Andrea Vanesa
Topa, Pascual Emilio
López Lastra, Claudia Cristina
Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
topic_facet Biología
Transverse Section
Entomopathogenic Fungus
Mosquito Larva
Fungal Structure
Histological Technique
description We evaluated which of the fixatives and stains most frequently used for observation of insect tissues were the most appropriate for histopathological visualization of entomopathogenic fungal infections with Leptolegnia chapmanii in larvae of Aedes aegypti. The best contrast between the host tissues and the fungal structures was obtained when using a combination of Camoy fixative with Grocott staining contrasted with light green. Masson trichromic stain combined with 10% formaldehyde-phosphate buffer also provided satisfactory results-a good contrast and clearly distinguishable host tissues and fungal structures.
format Articulo
Articulo
author Dikgolz, Vanesa Eva
Toledo, Andrea Vanesa
Topa, Pascual Emilio
López Lastra, Claudia Cristina
author_facet Dikgolz, Vanesa Eva
Toledo, Andrea Vanesa
Topa, Pascual Emilio
López Lastra, Claudia Cristina
author_sort Dikgolz, Vanesa Eva
title Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
title_short Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
title_full Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
title_fullStr Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
title_full_unstemmed Evaluation of histological techniques for the detection of fungal infections caused by Leptolegnia chapmanii (Oomycetes: Saprolegniales) in Aedes aegypti (Diptera: Culicidae) larvae
title_sort evaluation of histological techniques for the detection of fungal infections caused by leptolegnia chapmanii (oomycetes: saprolegniales) in aedes aegypti (diptera: culicidae) larvae
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/137558
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