Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay

An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for sele...

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Detalles Bibliográficos
Autores principales: Jones, Andy, Acquaviva, Agustín, Suktham, Thirada, Dennis, Gary R., Shalliker, R. Andrew, Soliven, Arianne
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137521
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137521
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
spellingShingle Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
Jones, Andy
Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
topic_facet Ciencias Exactas
Química
Total antioxidant capacity
Post column derivatisation
Reaction flow chromatography
Selective detection
Ferric reducing antioxidant power
description An established ferric reducing antioxidant power (FRAP) assay was optimised by preparation of the derivatisation reagent in 300 mM formate instead of 300 mM acetate conditions, resulting in increased sensitivity signal to noise responses by up to five to ten times. The quantitative protocol for selective detection of antioxidants via a HPLC post column derivatisation (PCD) technique using the 300 mM formate FRAP reagent conditions was then transformed into a high-speed qualitative screening protocol by utilizing an emerging technology ‘reaction flow (RF) chromatography’. Reaction flow chromatography’s ability to screen for total antioxidant capacity with additional peak specificity/profile information of active peaks could be achieved in under 2 min.
format Articulo
Articulo
author Jones, Andy
Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author_facet Jones, Andy
Acquaviva, Agustín
Suktham, Thirada
Dennis, Gary R.
Shalliker, R. Andrew
Soliven, Arianne
author_sort Jones, Andy
title Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_short Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_full Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_fullStr Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_full_unstemmed Total Antioxidant Capacity with Peak Specificity via Reaction Flow Chromatography and the Ferric Reducing Antioxidant Power Assay
title_sort total antioxidant capacity with peak specificity via reaction flow chromatography and the ferric reducing antioxidant power assay
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/137521
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