Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)

The fast-growing <i>Rhizobium</i> sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of <i>Sinorhizobium fredii</i>, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable ge...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Saldaña, Gustavo Fabián, Martínez Alcántara, Virginia, Vinardell, José María, Bellogín, Ramón A., Ruíz-Sainz, José E., Balatti, Pedro Alberto
Formato: Articulo
Lenguaje:Español
Publicado: 2003
Materias:
Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/137359
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-137359
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
spellingShingle Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
Saldaña, Gustavo Fabián
Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
topic_facet Ciencias Agrarias
Biología
Sinorhizobium fredii
Genotype
Genetic diversity
PCR
Symbiosis
Soybean
description The fast-growing <i>Rhizobium</i> sp. strain NGR234, isolated from Papua New Guinea, and 13 strains of <i>Sinorhizobium fredii</i>, isolated from China and Vietnam, were fingerprinted by means of RAPD, REP, ERIC and ARDRA. ERIC, REP and RAPD markers revealed a considerable genetic diversity among fast-growing rhizobia. Chinese isolates showed higher levels of diversity than those strains isolated from Vietnam. ARDRA analysis revealed three different genotypes among fast-growing rhizobia that nodulate soybean, even though all belonged to a subcluster that included <i>Sinorhizobium saheli</i> and <i>Sinorhizobium meliloti</i>. Among <i>S. fredii</i> rhizobia, two strains, SMH13 and HH303, might be representatives of other species of nitrogen-fixing organisms. Although restriction analysis of the <i>nifD–nifK</i> intergenic DNA fragment confirmed the unique nature of <i>Rhizobium</i> sp. strain NGR234, several similarities between <i>Rhizobium</i> sp. strain NGR234 and <i>S. fredii</i> USDA257, the ARDRA analysis and the full sequence of the 16S rDNA confirmed that NGR234 is a <i>S. fredii</i> strain. In addition, ARDRA analysis and the full sequence of the 16S rDNA suggested that two strains of rhizobia might be representatives of other species of rhizobia.
format Articulo
Articulo
author Saldaña, Gustavo Fabián
Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
author_facet Saldaña, Gustavo Fabián
Martínez Alcántara, Virginia
Vinardell, José María
Bellogín, Ramón A.
Ruíz-Sainz, José E.
Balatti, Pedro Alberto
author_sort Saldaña, Gustavo Fabián
title Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_short Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_full Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_fullStr Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_full_unstemmed Genetic diversity of fast-growing rhizobia that nodulate soybean (<i>Glycine max</i> L. Merr)
title_sort genetic diversity of fast-growing rhizobia that nodulate soybean (<i>glycine max</i> l. merr)
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/137359
work_keys_str_mv AT saldanagustavofabian geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
AT martinezalcantaravirginia geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
AT vinardelljosemaria geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
AT belloginramona geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
AT ruizsainzjosee geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
AT balattipedroalberto geneticdiversityoffastgrowingrhizobiathatnodulatesoybeaniglycinemaxilmerr
bdutipo_str Repositorios
_version_ 1764820456857141251

Ejemplares similares