Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy

The complex of vanadyl(IV) cation with oxodiacetate, VO(oda) caused an inhibitory effect on the proliferation of the human colon adenocarcinoma cell line Caco-2 in the range of 25–100 μM (P < 0.001). This inhibition was partially reversed by scavengers of free radicals. The difference in cell pro...

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Autores principales: Di Virgilio, Ana Laura, Rivadeneira, Josefina, Muglia, Cecilia Isabel, Reigosa, Miguel Antonio, Butenko, Nataliya, Cavaco, Isabel, Etcheverry, Susana Beatriz
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Ciencias Exactas
Bioquímica
Vanadyl(IV) cation
Multidentate ligands
Cytotoxicity
Genotoxicity
Caco-2 tumoral cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136691
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136691
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioquímica
Vanadyl(IV) cation
Multidentate ligands
Cytotoxicity
Genotoxicity
Caco-2 tumoral cells
spellingShingle Ciencias Exactas
Bioquímica
Vanadyl(IV) cation
Multidentate ligands
Cytotoxicity
Genotoxicity
Caco-2 tumoral cells
Di Virgilio, Ana Laura
Rivadeneira, Josefina
Muglia, Cecilia Isabel
Reigosa, Miguel Antonio
Butenko, Nataliya
Cavaco, Isabel
Etcheverry, Susana Beatriz
Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
topic_facet Ciencias Exactas
Bioquímica
Vanadyl(IV) cation
Multidentate ligands
Cytotoxicity
Genotoxicity
Caco-2 tumoral cells
description The complex of vanadyl(IV) cation with oxodiacetate, VO(oda) caused an inhibitory effect on the proliferation of the human colon adenocarcinoma cell line Caco-2 in the range of 25–100 μM (P < 0.001). This inhibition was partially reversed by scavengers of free radicals. The difference in cell proliferation in the presence and the absence of scavengers was statistically significant in the range of 50–100 μM (P < 0.05). VO(oda) altered lysosomal and mitochondria metabolisms (neutral red and MTT bioassays) in a dose–response manner from 10 μM (P < 0.001). Morphological studies showed important transformations that correlated with the disassembly of actin filaments and a decrease in the number of cells in a dose response manner. Moreover, VO(oda) caused statistically significant genotoxic effects on Caco-2 cells in the low range of concentration (5–25 μM) (Comet assay). Increment in the oxidative stress and a decrease in the GSH level are the main cytotoxic mechanisms of VO(oda). These effects were partially reversed by scavengers of free radicals in the range of 50–100 μM (P < 0.05). Besides, VO(oda) interacted with plasmidic DNA causing single and double strand cleavage, probably through the action of free radical species. Altogether, these results suggest that VO(oda) is a good candidate to be evaluated for alternative therapeutics in cancer treatment.
format Articulo
Articulo
author Di Virgilio, Ana Laura
Rivadeneira, Josefina
Muglia, Cecilia Isabel
Reigosa, Miguel Antonio
Butenko, Nataliya
Cavaco, Isabel
Etcheverry, Susana Beatriz
author_facet Di Virgilio, Ana Laura
Rivadeneira, Josefina
Muglia, Cecilia Isabel
Reigosa, Miguel Antonio
Butenko, Nataliya
Cavaco, Isabel
Etcheverry, Susana Beatriz
author_sort Di Virgilio, Ana Laura
title Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
title_short Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
title_full Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
title_fullStr Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
title_full_unstemmed Cyto- and genotoxicity of a vanadyl(IV) complex with oxodiacetate in human colon adenocarcinoma (Caco-2) cells: potential use in cancer therapy
title_sort cyto- and genotoxicity of a vanadyl(iv) complex with oxodiacetate in human colon adenocarcinoma (caco-2) cells: potential use in cancer therapy
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/136691
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