Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation

Soybean lectin (SBL) participates in the recognition between <i>Bradyrhizobium japonicum</i> and soybean although its role remains unknown. To search for changes in the proteome in response to SBL, <i>B. japonicum</i> USDA 110 was incubated for 12 h in a C- and N-free medium...

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Detalles Bibliográficos
Autores principales: Pérez Giménez, Julieta, Covelli, Julieta Mariana, López, María Florencia, Althabegoiti, María Julia, Ferrer-Navarro, Mario, Mongiardini, Elías Javier, Lodeiro, Aníbal Roberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Biología
Rhizobium
Bradyrhizobium japonicum
Infection Thread
Soluble Protein Fraction
Surface Polysaccharide
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136624
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136624
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Biología
Rhizobium
Bradyrhizobium japonicum
Infection Thread
Soluble Protein Fraction
Surface Polysaccharide
spellingShingle Ciencias Exactas
Biología
Rhizobium
Bradyrhizobium japonicum
Infection Thread
Soluble Protein Fraction
Surface Polysaccharide
Pérez Giménez, Julieta
Covelli, Julieta Mariana
López, María Florencia
Althabegoiti, María Julia
Ferrer-Navarro, Mario
Mongiardini, Elías Javier
Lodeiro, Aníbal Roberto
Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
topic_facet Ciencias Exactas
Biología
Rhizobium
Bradyrhizobium japonicum
Infection Thread
Soluble Protein Fraction
Surface Polysaccharide
description Soybean lectin (SBL) participates in the recognition between <i>Bradyrhizobium japonicum</i> and soybean although its role remains unknown. To search for changes in the proteome in response to SBL, <i>B. japonicum</i> USDA 110 was incubated for 12 h in a C- and N-free medium with or without SBL (10 μg ml ⁻¹), and the soluble protein profiles were compared. Two polypeptides, S-adenosyl-methionine synthetase (MetK) and the 30S ribosomal protein S1 (RpsA), were found only in the fractions from rhizobia incubated without SBL. Transcript levels of metK and rpsA were not correlated with polypeptide levels, indicating that there was regulation at translation. In support of this proposal, the 5′ translation initiation-region of rpsA mRNA contained folding elements as those involved in regulation of its translation in other species. Disappearance of MetK and RpsA from the soluble protein fractions of SBL-treated rhizobia suggests that SBL might have attenuated the nutritional stress response of <i>B. japonicum</i>.
format Articulo
Articulo
author Pérez Giménez, Julieta
Covelli, Julieta Mariana
López, María Florencia
Althabegoiti, María Julia
Ferrer-Navarro, Mario
Mongiardini, Elías Javier
Lodeiro, Aníbal Roberto
author_facet Pérez Giménez, Julieta
Covelli, Julieta Mariana
López, María Florencia
Althabegoiti, María Julia
Ferrer-Navarro, Mario
Mongiardini, Elías Javier
Lodeiro, Aníbal Roberto
author_sort Pérez Giménez, Julieta
title Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
title_short Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
title_full Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
title_fullStr Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
title_full_unstemmed Soybean Seed Lectin Prevents the Accumulation of S-Adenosyl Methionine Synthetase and the S1 30S Ribosomal Protein in <i>Bradyrhizobium japonicum</i> Under C and N Starvation
title_sort soybean seed lectin prevents the accumulation of s-adenosyl methionine synthetase and the s1 30s ribosomal protein in <i>bradyrhizobium japonicum</i> under c and n starvation
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/136624
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