Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats

Long-term diabetes mellitus can induce osteopenia and osteoporosis, an increase in the incidence of low-stress fractures, and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evalu...

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Autores principales: Alvarez-Lloret, Pedro, Fernández, Juan Manuel, Molinuevo, María Silvina, Lino, Agustina Berenice, Ferretti, José Luis, Capozza, Ricardo Francisco, Cortizo, Ana María, McCarthy, Antonio Desmond
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Diabetes Mellitus
Strontium ranelate
Bone mineralization
Microstructural properties
Bone biomechanics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136305
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136305
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Diabetes Mellitus
Strontium ranelate
Bone mineralization
Microstructural properties
Bone biomechanics
spellingShingle Biología
Diabetes Mellitus
Strontium ranelate
Bone mineralization
Microstructural properties
Bone biomechanics
Alvarez-Lloret, Pedro
Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
topic_facet Biología
Diabetes Mellitus
Strontium ranelate
Bone mineralization
Microstructural properties
Bone biomechanics
description Long-term diabetes mellitus can induce osteopenia and osteoporosis, an increase in the incidence of low-stress fractures, and/or delayed fracture healing. Strontium ranelate (SrR) is a dual-action anti-osteoporotic agent whose use in individuals with diabetic osteopathy has not been adequately evaluated. In this study, we studied the effects of an oral treatment with SrR and/or experimental diabetes on bone composition and biomechanics. Young male Wistar rats (half non-diabetic, half with streptozotocin/nicotinamide-induced diabetes) were either untreated or orally administered 625 mg/kg/day of SrR for 6 weeks. After sacrifice, femora from all animals were evaluated by a multi-scale approach (X-ray diffraction, Fourier transform infrared spectroscopy, inductively coupled plasma optical-emission spectrometry, static histomorphometry, pQCT, and mechanical testing) to determine chemical, crystalline, and biomechanical properties. Untreated diabetic animals (versus untreated non-diabetic) showed a decrease in femoral mineral carbonate content, in cortical thickness and BMC, in trabecular osteocyte density, in maximum load supported at rupture and at yield point, and in overall toughness at mid-shaft. Treatment of diabetic animals with SrR further affected several parameters of bone (some already impaired by diabetes): crystallinity index (indicating less mature apatite crystals); trabecular area, BMC, and vBMD; maximum load at yield point; and structural elastic rigidity. However, SrR was also able to prevent the diabetes-induced decreases in trabecular osteocyte density (completely) and in bone ultimate strength at rupture (partially). Our results indicate that SrR treatment can partially but significantly prevent some bone structural mechanical properties as previously affected by diabetes, but not others (which may even be worsened).
format Articulo
Articulo
author Alvarez-Lloret, Pedro
Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
author_facet Alvarez-Lloret, Pedro
Fernández, Juan Manuel
Molinuevo, María Silvina
Lino, Agustina Berenice
Ferretti, José Luis
Capozza, Ricardo Francisco
Cortizo, Ana María
McCarthy, Antonio Desmond
author_sort Alvarez-Lloret, Pedro
title Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
title_short Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
title_full Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
title_fullStr Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
title_full_unstemmed Multi-Scale Approach for the Evaluation of Bone Mineralization in Strontium Ranelate-Treated Diabetic Rats
title_sort multi-scale approach for the evaluation of bone mineralization in strontium ranelate-treated diabetic rats
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/136305
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