Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163

Toxoplasma gondii is an obligate intracellular protozoan parasite capable of infecting warm-blooded animals, including humans. A highly diverse genetic population has been reported in Central and South America, predominating mainly atypical genotypes. Different genotypes showed different biological...

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Autores principales: Bernstein, Mariana, Pardini, Lais Luján, Campero, Lucía María, Helman, María Elisa, Unzaga, Juan Manuel, Venturini, María Cecilia, Moré, Gastón Andrés
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Veterinarias
Biología
T. gondii
Atypical isolates
Invasion
Replication
Mouse virulence
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/136292
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-136292
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Biología
T. gondii
Atypical isolates
Invasion
Replication
Mouse virulence
spellingShingle Ciencias Veterinarias
Biología
T. gondii
Atypical isolates
Invasion
Replication
Mouse virulence
Bernstein, Mariana
Pardini, Lais Luján
Campero, Lucía María
Helman, María Elisa
Unzaga, Juan Manuel
Venturini, María Cecilia
Moré, Gastón Andrés
Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
topic_facet Ciencias Veterinarias
Biología
T. gondii
Atypical isolates
Invasion
Replication
Mouse virulence
description Toxoplasma gondii is an obligate intracellular protozoan parasite capable of infecting warm-blooded animals, including humans. A highly diverse genetic population has been reported in Central and South America, predominating mainly atypical genotypes. Different genotypes showed different biological behavior in mice. The aim of this study was to evaluate the biological behavior of T. gondii isolates obtained from Macropus rufogriseus (TgMr) and Saimiri boliviensis (TgSb) identified as atypical genotypes # 14 and # 163, respectively. Strains RH, ME49 and VEG were used as reference for clonal types I, II and III, respectively. In vitro invasion and replication capacity assays were analyzed at 6 and 18 hpi, respectively. In vivo assay was performed in Swiss mice (n = 30) using 1 × 102 and 1 × 103 parasites/mouse as infective doses (ME49, VEG, TgMr, TgSb and negative control). Morbi-mortality and tissues PCR were assessed. Lymphoproliferation assays were performed and gamma interferon was measured by ELISA. The ME49 strain showed the highest invasion, followed by TgSb and VEG, while RH and TgMr presented the lowest invasions. The RH strain and the TgSb isolate showed more endodyogeny events (fastest doubling times) than VEG and ME49 strains and the TgMr isolate. Both atypical isolates showed high virulence (100% morbi-mortality, at 8–10 dpi) and parasite DNA was detected in all tissue samples. Splenocytes from mice inoculated with TgMr and TgSb registered the highest values of gamma interferon. An in vitro invasion-replication index was established which correlates inversely with virulence in mice. In conclusion, T. gondii atypical isolates # 14 and # 163 showed a different in vitro behavior than clonal strains, with low invasion-replication indexes but being highly virulent in mouse model.
format Articulo
Preprint
author Bernstein, Mariana
Pardini, Lais Luján
Campero, Lucía María
Helman, María Elisa
Unzaga, Juan Manuel
Venturini, María Cecilia
Moré, Gastón Andrés
author_facet Bernstein, Mariana
Pardini, Lais Luján
Campero, Lucía María
Helman, María Elisa
Unzaga, Juan Manuel
Venturini, María Cecilia
Moré, Gastón Andrés
author_sort Bernstein, Mariana
title Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
title_short Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
title_full Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
title_fullStr Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
title_full_unstemmed Evaluation of biological behavior of Toxoplasma gondii atypical isolates # 14 and # 163
title_sort evaluation of biological behavior of toxoplasma gondii atypical isolates # 14 and # 163
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/136292
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