<i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production

In patients with active brucellosis, the liver is frequently affected by histopathologic lesions, such as granulomas, inflammatory infiltrations, and parenchymal necrosis. Herein, we examine some potential mechanisms of liver damage in brucellosis. We demonstrate that <i>Brucella abortus</i...

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Detalles Bibliográficos
Autores principales: Arriola Benitez, Paula Constanza, Scian, Romina, Comerci, Diego J., Rey Serantes, Diego A., Vanzulli, Silvia, Fossati, Carlos Alberto, Giambartolomei, Guillermo H., Delpino, M. Victoria
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Medicina
Biología
immunopathology
infectious diseases
brucellosis
Brucella abortus
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/135354
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-135354
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Medicina
Biología
immunopathology
infectious diseases
brucellosis
Brucella abortus
spellingShingle Medicina
Biología
immunopathology
infectious diseases
brucellosis
Brucella abortus
Arriola Benitez, Paula Constanza
Scian, Romina
Comerci, Diego J.
Rey Serantes, Diego A.
Vanzulli, Silvia
Fossati, Carlos Alberto
Giambartolomei, Guillermo H.
Delpino, M. Victoria
<i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
topic_facet Medicina
Biología
immunopathology
infectious diseases
brucellosis
Brucella abortus
description In patients with active brucellosis, the liver is frequently affected by histopathologic lesions, such as granulomas, inflammatory infiltrations, and parenchymal necrosis. Herein, we examine some potential mechanisms of liver damage in brucellosis. We demonstrate that <i>Brucella abortus</i> infection inhibits matrix metalloproteinase-9 (MMP-9) secretion and induces collagen deposition and tissue inhibitor of matrix metalloproteinase-1 secretion induced by hepatic stellate cells (LX-2). These phenomena depend on transforming growth factor-β1 induction. In contrast, supernatants from <i>B. abortus</i> –infected hepatocytes and monocytes induce MMP-9 secretion and inhibit collagen deposition in hepatic stellate cells. Yet, if LX-2 cells are infected with <i>B. abortus</i>, the capacity of supernatants from <i>B. abortus</i> –infected hepatocytes and monocytes to induce MMP-9 secretion and inhibit collagen deposition is abrogated. These results indicate that depending on the balance between interacting cells and cytokines of the surrounding milieu, the response of LX-2 cells could be turned into an inflammatory or fibrogenic phenotype. Livers from mice infected with <i>B. abortus</i> displayed a fibrogenic phenotype with patches of collagen deposition and transforming growth factor-β1 induction. This study provides potential mechanisms of liver immune response induced by <i>B. abortus</i> –infected hepatic stellate cells. In addition, these results demonstrate that the cross talk of these cells with hepatocytes and macrophages implements a series of interactions that may contribute to explaining some of mechanisms of liver damage observed in human brucellosis.
format Articulo
Articulo
author Arriola Benitez, Paula Constanza
Scian, Romina
Comerci, Diego J.
Rey Serantes, Diego A.
Vanzulli, Silvia
Fossati, Carlos Alberto
Giambartolomei, Guillermo H.
Delpino, M. Victoria
author_facet Arriola Benitez, Paula Constanza
Scian, Romina
Comerci, Diego J.
Rey Serantes, Diego A.
Vanzulli, Silvia
Fossati, Carlos Alberto
Giambartolomei, Guillermo H.
Delpino, M. Victoria
author_sort Arriola Benitez, Paula Constanza
title <i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
title_short <i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
title_full <i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
title_fullStr <i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
title_full_unstemmed <i>Brucella abortus</i> Induces Collagen Deposition and MMP-9 Down-Modulation in Hepatic Stellate Cells via TGF-β1 Production
title_sort <i>brucella abortus</i> induces collagen deposition and mmp-9 down-modulation in hepatic stellate cells via tgf-β1 production
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/135354
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