Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.

Scatchard analysis of prolactin binding sites (PRL-BS) from ejaculated spermatozoa showed a single population of binding sites (apparent association constant: 2.51+/-0.186 nmol/l[-1]) with 0.317+/-0.0743 fmol/10(6) sperm binding sites. Different pools of spermatozoa were incubated with increasing co...

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Detalles Bibliográficos
Autores principales: Luthy, I. A., Mormandi, E., Aszpis, S., Vázquez, Stella Maris, Maccallini, G., Levalle, O., Calandra, Ricardo Saúl
Formato: Articulo
Lenguaje:Inglés
Publicado: 1997
Materias:
Ciencias Médicas
Prolactin receptor
spermatozoa
asthenospermic
normospermic
reproduction
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/134784
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-134784
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Prolactin receptor
spermatozoa
asthenospermic
normospermic
reproduction
spellingShingle Ciencias Médicas
Prolactin receptor
spermatozoa
asthenospermic
normospermic
reproduction
Luthy, I. A.
Mormandi, E.
Aszpis, S.
Vázquez, Stella Maris
Maccallini, G.
Levalle, O.
Calandra, Ricardo Saúl
Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
topic_facet Ciencias Médicas
Prolactin receptor
spermatozoa
asthenospermic
normospermic
reproduction
description Scatchard analysis of prolactin binding sites (PRL-BS) from ejaculated spermatozoa showed a single population of binding sites (apparent association constant: 2.51+/-0.186 nmol/l[-1]) with 0.317+/-0.0743 fmol/10(6) sperm binding sites. Different pools of spermatozoa were incubated with increasing concentrations of several hormones. There was a decrease in [125I]-oPRL binding with purified ovine prolactin (oPRL) and human growth hormone (hGH) which was not observed in the presence of synthetic ACTH and recombinant FSH, suggesting that binding was hormone specific. When the patient's samples were analyzed using the single point assay at saturation concentration, asthenospermic patients showed a significantly higher concentration of binding sites compared to normospermic ones. Both groups of patients displayed similar PRL levels in seminal plasma measured by DELFIA. Moreover, individual values of PRL levels in seminal plasma did not correlate with PRL-BS concentrations. We thus conclude that [125I]-oPRL binding to ejaculated spermatozoa was hormone specific and with similar parameters as seen in other target tissues. PRL-BS concentration in asthenospermic patients was significantly higher than in normospermic but this was not due to different levels of PRL in seminal plasma.
format Articulo
Articulo
author Luthy, I. A.
Mormandi, E.
Aszpis, S.
Vázquez, Stella Maris
Maccallini, G.
Levalle, O.
Calandra, Ricardo Saúl
author_facet Luthy, I. A.
Mormandi, E.
Aszpis, S.
Vázquez, Stella Maris
Maccallini, G.
Levalle, O.
Calandra, Ricardo Saúl
author_sort Luthy, I. A.
title Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
title_short Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
title_full Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
title_fullStr Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
title_full_unstemmed Binding of <SUP>125</SUP>I-prolactin to spermatozoa from normospermic and asthenospermic men.
title_sort binding of <sup>125</sup>i-prolactin to spermatozoa from normospermic and asthenospermic men.
publishDate 1997
url http://sedici.unlp.edu.ar/handle/10915/134784
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