Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>

The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of t...

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Autores principales:	Guiñazú, Lorena Belén, Andrés, Javier Alberto, Del Papa, María Florencia, Pistorio, Mariano, Rosas, Susana Beatriz
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Biología alfalfa Plant growth-promoting rhizobacteria Coinoculation Biological nitrogen fixation
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/134058
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-134058
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología alfalfa Plant growth-promoting rhizobacteria Coinoculation Biological nitrogen fixation
spellingShingle	Biología alfalfa Plant growth-promoting rhizobacteria Coinoculation Biological nitrogen fixation Guiñazú, Lorena Belén Andrés, Javier Alberto Del Papa, María Florencia Pistorio, Mariano Rosas, Susana Beatriz Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
topic_facet	Biología alfalfa Plant growth-promoting rhizobacteria Coinoculation Biological nitrogen fixation
description	The objectives of this work were to phenotypically and genetically characterize alfalfa rhizosphere bacteria and to evaluate the effect of single or mixed inoculation upon nodulation and biological nitrogen fixation. Thirty-two strains showed tricalcium phosphate solubilization ability, and two of them caused bigger or equal solubilization halos than the control strain <i>P. putida</i> SP22. The comparison of the 16S ribosomal DNA sequences indicated that these strains are phylogenetically related to <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. A beneficial effect of both isolates on alfalfa growth was observed in coinoculation assays. <i>Pseudomonas</i> sp. FM7d caused a significant increase in root and shoot dry weight, length, and surface area of roots, number, and symbiotic properties of alfalfa plants. The plants coinoculated with <i>Sinorhizobium meliloti</i> B399 and the <i>Bacillus</i> sp. M7c showed significant increases in the measured parameters. Our results indicating that strains <i>Pseudomonas</i> sp. FM7d and <i>Bacillus</i> sp. M7c can be considered for the formulation of new inoculants.
format	Articulo Articulo
author	Guiñazú, Lorena Belén Andrés, Javier Alberto Del Papa, María Florencia Pistorio, Mariano Rosas, Susana Beatriz
author_facet	Guiñazú, Lorena Belén Andrés, Javier Alberto Del Papa, María Florencia Pistorio, Mariano Rosas, Susana Beatriz
author_sort	Guiñazú, Lorena Belén
title	Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_short	Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_full	Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_fullStr	Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_full_unstemmed	Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>
title_sort	response of alfalfa (<i>medicago sativa</i> l.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>sinorhizobium meliloti</i>
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/134058
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bdutipo_str	Repositorios
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Response of alfalfa (<i>Medicago sativa</i> L.) to single and mixed inoculation with phosphate-solubilizing bacteria and <i>Sinorhizobium meliloti</i>

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