Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication

Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternati...

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Autores principales:	Soler Arango, Juliana, Xaubet, Magalí, Giuliani, Leandro, Grondona, Diana, Brelles Mariño, Graciela
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2017
Materias:	Química biofilms Pseudomonas aeruginosa sterilization biofilm eradication nonthermal plasmas air-based plasma Pseudomonas biofilms
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/133961
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-133961
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química biofilms Pseudomonas aeruginosa sterilization biofilm eradication nonthermal plasmas air-based plasma Pseudomonas biofilms
spellingShingle	Química biofilms Pseudomonas aeruginosa sterilization biofilm eradication nonthermal plasmas air-based plasma Pseudomonas biofilms Soler Arango, Juliana Xaubet, Magalí Giuliani, Leandro Grondona, Diana Brelles Mariño, Graciela Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
topic_facet	Química biofilms Pseudomonas aeruginosa sterilization biofilm eradication nonthermal plasmas air-based plasma Pseudomonas biofilms
description	Biofilms cause biofouling, pipe plugging, prostheses colonization, disease, and nosocomial infections. Bacterial biofilms are more resilient to sterilization methods than planktonic bacteria; therefore, better control methods are required. The use of gas discharge plasmas is an appropriate alternative because plasmas contain a mixture of reactive agents that are well known for bacterial decontamination. This study assesses culture medium-abiotic surface combinations leading to robust biofilms and tests an air-based coaxial dielectric barrier discharge (DBD) plasma source on Pseudomonas aeruginosa biofilms grown in continuous culture under those selected conditions. Biofilms were eradicated after a 15-min plasma treatment, resulting in a CFU/mL decrease of 5.6 log10 units. CFU/mL decreases of 1.6 and 2.7 log10 units were achieved after a 3-min plasma exposure to ambient and moistened air plasma, respectively, although viability assays showed that some cells were alive. Moistened-air plasma resulted in a faster biofilm inactivation, with decimal reduction times of 1.14 and 4.36 min. The coaxial DBD air-based plasma source presented here is effective for Pseudomonas biofilm inactivation, affordable because it does not rely on expensive gases, and easy to handle for indirect surface treatment. To the best of our knowledge, the search for the best combination medium surface leading to robust biofilms before plasma treatment has not been previously assessed.
format	Articulo Articulo
author	Soler Arango, Juliana Xaubet, Magalí Giuliani, Leandro Grondona, Diana Brelles Mariño, Graciela
author_facet	Soler Arango, Juliana Xaubet, Magalí Giuliani, Leandro Grondona, Diana Brelles Mariño, Graciela
author_sort	Soler Arango, Juliana
title	Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
title_short	Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
title_full	Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
title_fullStr	Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
title_full_unstemmed	Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication
title_sort	air-based coaxial dielectric barrier discharge plasma source for <i>pseudomonas aeruginosa</i> biofilm eradication
publishDate	2017
url	http://sedici.unlp.edu.ar/handle/10915/133961
work_keys_str_mv	AT solerarangojuliana airbasedcoaxialdielectricbarrierdischargeplasmasourceforipseudomonasaeruginosaibiofilmeradication AT xaubetmagali airbasedcoaxialdielectricbarrierdischargeplasmasourceforipseudomonasaeruginosaibiofilmeradication AT giulianileandro airbasedcoaxialdielectricbarrierdischargeplasmasourceforipseudomonasaeruginosaibiofilmeradication AT grondonadiana airbasedcoaxialdielectricbarrierdischargeplasmasourceforipseudomonasaeruginosaibiofilmeradication AT brellesmarinograciela airbasedcoaxialdielectricbarrierdischargeplasmasourceforipseudomonasaeruginosaibiofilmeradication
bdutipo_str	Repositorios
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Air-Based Coaxial Dielectric Barrier Discharge Plasma Source for <i>Pseudomonas aeruginosa</i> Biofilm Eradication

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