Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice

Growth hormone secretagogue receptor (GHSR), the receptor for ghrelin, is expressed in key brain nuclei that regulate food intake. The dopamine (DA) pathways have long been recognized to play key roles mediating GHSR effects on feeding behaviors. Here, we aimed to determine the role of GHSR in DA ne...

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Autores principales:	Cornejo, María Paula, Barrile, Franco, Cassano, Daniela Alejandra, Aguggia, Julieta Paola, Romero, Guadalupe Garcia, Reynaldo, Mirta Beatriz, Andreoli, Maria Florencia, De Francesco, Pablo Nicolás, Perelló, Mario Carlos
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2020
Materias:	Medicina GHSR dopamine high-fat mesocorticolimbic pathway ghrelin appetite
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/133814
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-133814
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Medicina GHSR dopamine high-fat mesocorticolimbic pathway ghrelin appetite
spellingShingle	Medicina GHSR dopamine high-fat mesocorticolimbic pathway ghrelin appetite Cornejo, María Paula Barrile, Franco Cassano, Daniela Alejandra Aguggia, Julieta Paola Romero, Guadalupe Garcia Reynaldo, Mirta Beatriz Andreoli, Maria Florencia De Francesco, Pablo Nicolás Perelló, Mario Carlos Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
topic_facet	Medicina GHSR dopamine high-fat mesocorticolimbic pathway ghrelin appetite
description	Growth hormone secretagogue receptor (GHSR), the receptor for ghrelin, is expressed in key brain nuclei that regulate food intake. The dopamine (DA) pathways have long been recognized to play key roles mediating GHSR effects on feeding behaviors. Here, we aimed to determine the role of GHSR in DA neurons controlling appetitive and consummatory behaviors towards high fat (HF) diet. For this purpose, we crossed reactivable GHSR-deficient mice with DA transporter (DAT)-Cre mice, which express Cre recombinase under the DAT promoter that is active exclusively in DA neurons, to generate mice with GHSR expression limited to DA neurons (DAT-GHSR mice). We found that DAT-GHSR mice show an increase of c-Fos levels in brain areas containing DA neurons after ghrelin treatment, in a similar fashion as seen in wild-type mice; however, they did not increase food intake or locomotor activity in response to systemically- or centrally-administered ghrelin. In addition, we found that satiated DAT-GHSR mice displayed both anticipatory activity to scheduled HF diet exposure and HF intake in a binge-like eating protocol similar to those in wild-type mice, whereas GHSR-deficient mice displayed impaired responses. We conclude that GHSR expression in DA neurons is sufficient to both mediate increased anticipatory activity to a scheduled HF diet exposure and fully orchestrate binge-like HF intake, but it is insufficient to restore the acute orexigenic or locomotor effects of ghrelin treatment. Thus, GHSR in DA neurons affects appetitive and consummatory behaviors towards HF diet that take place in the absence of caloric needs.
format	Articulo Preprint
author	Cornejo, María Paula Barrile, Franco Cassano, Daniela Alejandra Aguggia, Julieta Paola Romero, Guadalupe Garcia Reynaldo, Mirta Beatriz Andreoli, Maria Florencia De Francesco, Pablo Nicolás Perelló, Mario Carlos
author_facet	Cornejo, María Paula Barrile, Franco Cassano, Daniela Alejandra Aguggia, Julieta Paola Romero, Guadalupe Garcia Reynaldo, Mirta Beatriz Andreoli, Maria Florencia De Francesco, Pablo Nicolás Perelló, Mario Carlos
author_sort	Cornejo, María Paula
title	Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
title_short	Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
title_full	Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
title_fullStr	Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
title_full_unstemmed	Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
title_sort	growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/133814
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Growth hormone secretagogue receptor in dopamine neurons controls appetitive and consummatory behaviors towards high-fat diet in ad-libitum fed mice

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