Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths

Light of different wavelengths can be used to obtain a more profitable outcome of photodynamic therapy (PDT), according to the absorption bands of the photosensitizer (PS). Low-grade cervical intraepithelial neoplasias (CINs) are superficial lesions that can be treated with light of shorter waveleng...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Etcheverry, María Eugenia, Pasquale, Miguel Ángel, Bergna, Cecilia, Ponzinibbio, Carlos, Garavaglia, Mario José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Física
Ciencias Médicas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/133626
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-133626
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Física
Ciencias Médicas
spellingShingle Física
Ciencias Médicas
Etcheverry, María Eugenia
Pasquale, Miguel Ángel
Bergna, Cecilia
Ponzinibbio, Carlos
Garavaglia, Mario José
Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
topic_facet Física
Ciencias Médicas
description Light of different wavelengths can be used to obtain a more profitable outcome of photodynamic therapy (PDT), according to the absorption bands of the photosensitizer (PS). Low-grade cervical intraepithelial neoplasias (CINs) are superficial lesions that can be treated with light of shorter wavelength than red because a large light penetration depth in tissue is not necessary. We report a comparative investigation performed to evaluate the efficacy of light-emitting diodes (LEDs) of different wavelengths in the photodynamic treatment applied to both 2D and 3D HeLa cell spheroid cultures. The spheroids are utilized as a PDT dosage model, and cell viability is evaluated at different sections of the spheroids by confocal microscopy. Cells incubated with m-tetrahydroxyphenyl chlorin are illuminated with LED systems working in the low fluence range, emitting in the violet (390–415nm), blue (440–470nm), red (620–645nm) and deep red (640–670nm) regions of the light spectrum at various exposures times (tI) comprised between 0.5 and 30min. PDT experiments performed on both 2D and 3D cell cultures indicate that the PDT treatment outcome is more efficient with violet light followed by red light. Dynamic data from the front displacement velocity of large 2D-quasi-radial colonies generated from cell spheroids adhered to the Petri dish bottom as well as the evolution of the 3D growth give further insight about the effect of PDT at each condition. Results from 3D cultures indicate that the penetration of the violet light is appropriate to kill HeLa cells several layers below, showing cell damage and death not only in the outer rim of the illuminated spheroids, where a PS accumulation exists, but also in the more internal region. Results indicate that violet LED light could be useful to treat CINs involving superficial dysplasia.
format Articulo
Articulo
author Etcheverry, María Eugenia
Pasquale, Miguel Ángel
Bergna, Cecilia
Ponzinibbio, Carlos
Garavaglia, Mario José
author_facet Etcheverry, María Eugenia
Pasquale, Miguel Ángel
Bergna, Cecilia
Ponzinibbio, Carlos
Garavaglia, Mario José
author_sort Etcheverry, María Eugenia
title Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
title_short Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
title_full Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
title_fullStr Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
title_full_unstemmed Photodynamic therapy in 2D and 3D human cervical carcinoma cell cultures employing LED light sources emitting at different wavelengths
title_sort photodynamic therapy in 2d and 3d human cervical carcinoma cell cultures employing led light sources emitting at different wavelengths
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/133626
work_keys_str_mv AT etcheverrymariaeugenia photodynamictherapyin2dand3dhumancervicalcarcinomacellculturesemployingledlightsourcesemittingatdifferentwavelengths
AT pasqualemiguelangel photodynamictherapyin2dand3dhumancervicalcarcinomacellculturesemployingledlightsourcesemittingatdifferentwavelengths
AT bergnacecilia photodynamictherapyin2dand3dhumancervicalcarcinomacellculturesemployingledlightsourcesemittingatdifferentwavelengths
AT ponzinibbiocarlos photodynamictherapyin2dand3dhumancervicalcarcinomacellculturesemployingledlightsourcesemittingatdifferentwavelengths
AT garavagliamariojose photodynamictherapyin2dand3dhumancervicalcarcinomacellculturesemployingledlightsourcesemittingatdifferentwavelengths
bdutipo_str Repositorios
_version_ 1764820455106019328

Ejemplares similares