Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation

Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized nu...

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Autores principales: Gómez-Zavaglia, Andrea, Kociubinski, Guillermo Luis, Pérez, Pablo Fernando, De Antoni, Graciela Liliana
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Química
Bifidobacterium
Probiotic
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/132489
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-132489
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Bifidobacterium
Probiotic
spellingShingle Química
Bifidobacterium
Probiotic
Gómez-Zavaglia, Andrea
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
topic_facet Química
Bifidobacterium
Probiotic
description Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.
format Articulo
Articulo
author Gómez-Zavaglia, Andrea
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_facet Gómez-Zavaglia, Andrea
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_sort Gómez-Zavaglia, Andrea
title Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_short Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_full Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_fullStr Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_full_unstemmed Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_sort isolation and characterization of bifidobacterium strains for probiotic formulation
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/132489
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