Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat

Systolic blood pressure (SBP) is frequently measured in rats by the tail cuff method, which usually comprises pulse/flow disappearance and reappearance during cuff inflation (Inf) and deflation (Def), separated by an interval between cycles (IBC). Although Def values are habitually used to estimate...

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Autores principales: Fritz, Mariana, Rinaldi, Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2007
Materias:
Ciencias Médicas
L-NAME
Systolic blood pressure
Nitric oxide
Tail-cuff method
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/132487
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-132487
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
L-NAME
Systolic blood pressure
Nitric oxide
Tail-cuff method
spellingShingle Ciencias Médicas
L-NAME
Systolic blood pressure
Nitric oxide
Tail-cuff method
Fritz, Mariana
Rinaldi, Gustavo
Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
topic_facet Ciencias Médicas
L-NAME
Systolic blood pressure
Nitric oxide
Tail-cuff method
description Systolic blood pressure (SBP) is frequently measured in rats by the tail cuff method, which usually comprises pulse/flow disappearance and reappearance during cuff inflation (Inf) and deflation (Def), separated by an interval between cycles (IBC). Although Def values are habitually used to estimate SBP, in 58 Wistar rats we found (Def–Inf) to be −6 ± 1 mmHg, indicating that Def < Inf in most cases. When the IBC was lengthened to 2 min, (Def–Inf) was increased to −17 ± 2 mmHg, indicating the probable accumulation of a vasodilating metabolite. This increase of (Def–Inf) was prevented by papaverine, indicating its relation to smooth muscle contractility. Adrenergic blockade did not prevent the increase of (Def–Inf), but pretreatment with L-NAME decreased it to −5 ± 2 mmHg (p < 0.05). Simultaneous measurement of SBP by tail-cuff method and carotid cannulation revealed that the Inf value was the most accurate estimation of intravascular SBP. We conclude that: (1) the Inf value should be taken as representative of SBP, since depending on the duration of suprasystolic compression the Def value can underestimate it, and (2) nitric oxide accumulation due to flow deprivation was the main cause of SBP underestimation by Def values.
format Articulo
Articulo
author Fritz, Mariana
Rinaldi, Gustavo
author_facet Fritz, Mariana
Rinaldi, Gustavo
author_sort Fritz, Mariana
title Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
title_short Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
title_full Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
title_fullStr Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
title_full_unstemmed Influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
title_sort influence of nitric oxide-mediated vasodilation on the blood pressure measured with the tail-cuff method in the rat
publishDate 2007
url http://sedici.unlp.edu.ar/handle/10915/132487
work_keys_str_mv AT fritzmariana influenceofnitricoxidemediatedvasodilationonthebloodpressuremeasuredwiththetailcuffmethodintherat
AT rinaldigustavo influenceofnitricoxidemediatedvasodilationonthebloodpressuremeasuredwiththetailcuffmethodintherat
bdutipo_str Repositorios
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