Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages

The expression of preproinsulin (ppIns), proglucagon, prosomatostatin, and propancreatic polypeptide was investigated in thymic extracts, thymic cells, and thymic cell lines from C57BL/6 mice by RT-PCR. The expression of pancreatic hormones was similar in thymic extracts taken from neonatal and 2-,...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Throsby, Mark, Homo-Delarche, Françoise, Chevenne, Didier, Goya, Rodolfo Gustavo, Dardenne, Mireille, Pléau, Jean-Marie
Formato: Articulo
Lenguaje:Inglés
Publicado: 1998
Materias:
Ciencias Exactas
Química
pancreatic hormones
mice
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131986
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131986
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Química
pancreatic hormones
mice
spellingShingle Ciencias Exactas
Química
pancreatic hormones
mice
Throsby, Mark
Homo-Delarche, Françoise
Chevenne, Didier
Goya, Rodolfo Gustavo
Dardenne, Mireille
Pléau, Jean-Marie
Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
topic_facet Ciencias Exactas
Química
pancreatic hormones
mice
description The expression of preproinsulin (ppIns), proglucagon, prosomatostatin, and propancreatic polypeptide was investigated in thymic extracts, thymic cells, and thymic cell lines from C57BL/6 mice by RT-PCR. The expression of pancreatic hormones was similar in thymic extracts taken from neonatal and 2-, 4-, and 8-week-old animals, but was decreased in 20-week-old animals. Pancreatic hormone expression was not observed in mouse liver, salivary gland, or spleen. Analysis of thymic cell populations revealed a 10- to 20-fold enrichment in expression of all hormones in low buoyant density cells. No expression was detected in high buoyant density cells (predominantly thymocytes) or in thymic epithelial cell lines, primary cultures of epithelial cells, or peripheral macrophages. In addition, immunoreactive insulin, measured by specific RIA, was detectable in the low buoyant density population, but not in high buoyant density cells. The enriched cell population was depleted of contaminating lymphocytes and sorted based on reactivity to the cell surface markers F4/80 (macrophage) or N418 (dendritic cells). Cells gated for N418 demonstrated expression for ppIns, but not the other pancreatic hormones. Conversely, expression for proglucagon, prosomatostatin, and propancreatic polypeptide, but not ppIns, was detected in F4/80-gated cells. Our data indicate that pancreatic endocrine hormones are differentially expressed by dendritic cells and macrophages in a normal mice.
format Articulo
Articulo
author Throsby, Mark
Homo-Delarche, Françoise
Chevenne, Didier
Goya, Rodolfo Gustavo
Dardenne, Mireille
Pléau, Jean-Marie
author_facet Throsby, Mark
Homo-Delarche, Françoise
Chevenne, Didier
Goya, Rodolfo Gustavo
Dardenne, Mireille
Pléau, Jean-Marie
author_sort Throsby, Mark
title Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
title_short Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
title_full Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
title_fullStr Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
title_full_unstemmed Pancreatic Hormone Expression in the Murine Thymus : Localization in Dendritic Cells and Macrophages
title_sort pancreatic hormone expression in the murine thymus : localization in dendritic cells and macrophages
publishDate 1998
url http://sedici.unlp.edu.ar/handle/10915/131986
work_keys_str_mv AT throsbymark pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
AT homodelarchefrancoise pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
AT chevennedidier pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
AT goyarodolfogustavo pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
AT dardennemireille pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
AT pleaujeanmarie pancreatichormoneexpressioninthemurinethymuslocalizationindendriticcellsandmacrophages
bdutipo_str Repositorios
_version_ 1764820453757550593

Ejemplares similares