Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene

The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and...

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Detalles Bibliográficos
Autores principales: Dewey, Ricardo Alfredo, Semorile, Liliana Carmen, Crisci, Jorge Víctor, Grau, Oscar
Formato: Articulo
Lenguaje:Inglés
Publicado: 1996
Materias:
Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131981
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131981
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
spellingShingle Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
Dewey, Ricardo Alfredo
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
topic_facet Bioquímica
Tospovirus
N gene
RT-PCR
Cladistics
description The genomic diversity of Argentine Tospoviruses from different geographical areas, and from several distinct crops was analysed here. For each isolate, RT-PCR, cloning and sequencing of a 450 nt fragment of the N gene were performed. Comparisons of RNA and predicted amino acid sequence identity and similarity were made. A partial sequence of the N gene was able to classify our local isolates within three Tospovirus species previously described (Tomato spotted wilt virus, TSWV; tomato chlorotic spot virus, TCSV and groundnut ringspot virus, GRSV). With the sequence data currently available, we performed a cladistic phylogenetic analysis which gave a possible genealogy among members of the Tospovirus genus.
format Articulo
Articulo
author Dewey, Ricardo Alfredo
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
author_facet Dewey, Ricardo Alfredo
Semorile, Liliana Carmen
Crisci, Jorge Víctor
Grau, Oscar
author_sort Dewey, Ricardo Alfredo
title Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_short Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_full Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_fullStr Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_full_unstemmed Clustering of Argentinean Tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide RNA region of the N gene
title_sort clustering of argentinean tospoviruses with existing species in the genus by sequence analysis of a 450-nucleotide rna region of the n gene
publishDate 1996
url http://sedici.unlp.edu.ar/handle/10915/131981
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