Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina

The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to...

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Autores principales: Alippi, Adriana Mónica, López, Ana Claudia, Balatti, Pedro Alberto
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131705
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131705
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
spellingShingle Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
Alippi, Adriana Mónica
López, Ana Claudia
Balatti, Pedro Alberto
Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
topic_facet Botánica
Agrobacterium rubi
Agrobacterium tumefaciens
Agrobacterium rhizogenes
Biovars
Vaccinium corymbossum
Blueberries
Crown gall disease
Diversity
PCR
description The aim of this study was to isolate, identify and analyze the diversity of the causative agents of crown galls and hairy roots from symptomatic plants of Vaccinium corymbossum by means of biological, biochemical and molecular tools. All the bacteria isolated from blueberries (n = 78) were found to be Agrobacterium since they grew on three differential media, provoked cell and/or root proliferation on Kalanchoe, and contained a 730 bp partial sequence that codes for virulence genes within the virC operon found on Ti and/or Ri plasmids. Isolates were highly variable considering the ERIC-PCR patterns as well as biochemical reactions and were all represented by 7 different restriction patterns of the 16SrDNA. While most of the isolates belonged to Agrobacterium bv. 1 (n = 33) or Agrobacterium bv. 2 (n = 31) only fourteen were Agrobacterium rubi. A representative isolate of each of these three groups was further identified by sequencing the approximately 400 bp 16SrDNA. We concluded that Vaccinium plants are particularly susceptible to Agrobacterium bv. 1, Agrobacterium bv. 2, and also to Agrobacterium rubi. To our knowledge this is the first survey of Agrobacterium affecting blueberries in Argentina.
format Articulo
Articulo
author Alippi, Adriana Mónica
López, Ana Claudia
Balatti, Pedro Alberto
author_facet Alippi, Adriana Mónica
López, Ana Claudia
Balatti, Pedro Alberto
author_sort Alippi, Adriana Mónica
title Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_short Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_full Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_fullStr Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_full_unstemmed Diversity among agrobacteria isolated from diseased plants of blueberry (Vaccinium corymbosum) in Argentina
title_sort diversity among agrobacteria isolated from diseased plants of blueberry (vaccinium corymbosum) in argentina
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/131705
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