Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis

The glucose carbon fluxes in continuous cultures of Bacillus thuringiensis grown in a complex medium have been studied as a function of the growth rate. The results are discussed in the light of a growth model. From reduced nicotinamide adenine dinucleotide (NADH) and carbon balances it was determin...

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Detalles Bibliográficos
Autores principales: Mignone, Carlos Fernando, Avignone Rossa, Claudio Adolfo
Formato: Articulo
Lenguaje:Inglés
Publicado: 1996
Materias:
Biología
Biomass
NADH
Adenine
Dinucleotide
Respiratory Chain
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/131364
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-131364
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Biomass
NADH
Adenine
Dinucleotide
Respiratory Chain
spellingShingle Biología
Biomass
NADH
Adenine
Dinucleotide
Respiratory Chain
Mignone, Carlos Fernando
Avignone Rossa, Claudio Adolfo
Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
topic_facet Biología
Biomass
NADH
Adenine
Dinucleotide
Respiratory Chain
description The glucose carbon fluxes in continuous cultures of Bacillus thuringiensis grown in a complex medium have been studied as a function of the growth rate. The results are discussed in the light of a growth model. From reduced nicotinamide adenine dinucleotide (NADH) and carbon balances it was determined that the fraction of glucose consumed for biomass synthesis decreased with the growth rate, while the glucose flux through the tricarboxylic acid (TCA) cycle diminished after a threshold value of D=0.34 h-1, where D=dilution rate. At the highest growth rate tested, glucose was used almost exclusively as the energy source, via fermentative pathways, which indicates that the yeast extract was used as the carbon source. The specific rate of oxygen consumption increased with growth even after the beginning of the accumulation of acids, indicating that the respiratory chain was not saturated. The results suggest that there is a mismatch between glycolysis and TCA cycle capacity, depending on the growth rate. Furthermore, values of (P/O) ratio and mATP are presented, where (P/O) is mole of ATP formed per gram atom oxygen consumed by the respiratory chains and mATP is the maintenance requirement for ATP.
format Articulo
Articulo
author Mignone, Carlos Fernando
Avignone Rossa, Claudio Adolfo
author_facet Mignone, Carlos Fernando
Avignone Rossa, Claudio Adolfo
author_sort Mignone, Carlos Fernando
title Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
title_short Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
title_full Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
title_fullStr Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
title_full_unstemmed Analysis of glucose carbon fluxes in continuous cultures of Bacillus thuringiensis
title_sort analysis of glucose carbon fluxes in continuous cultures of bacillus thuringiensis
publishDate 1996
url http://sedici.unlp.edu.ar/handle/10915/131364
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AT avignonerossaclaudioadolfo analysisofglucosecarbonfluxesincontinuousculturesofbacillusthuringiensis
bdutipo_str Repositorios
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