Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes

The aim of this study was to evaluate the effect of riboflavin supplementation of culture medium on conidial UV-A tolerance of M. acridum (Driver & Milner) (Hypocreales: Clavicipitaceae) and M. robertsii (Bischoff, Rehner & Humber) (Hypocreales: Clavicipitaceae). These fungi were produced on...

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Autores principales:	Huarte Bonnet, Carla, Pereira Junior, Ronaldo Alves, Santos da Paixão, Flávia Regina, Braga, Gilberto U.L., Roberts, Donald W., Luz, Christian, Pedrini, Nicolás, Fernandes, Éverton K. K.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Biología Biological control Entomopathogenic fungi Fungal UV tolerance Photoprotection Photoreactivation Vitamin B2
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/130954
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-130954
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Biological control Entomopathogenic fungi Fungal UV tolerance Photoprotection Photoreactivation Vitamin B2
spellingShingle	Biología Biological control Entomopathogenic fungi Fungal UV tolerance Photoprotection Photoreactivation Vitamin B2 Huarte Bonnet, Carla Pereira Junior, Ronaldo Alves Santos da Paixão, Flávia Regina Braga, Gilberto U.L. Roberts, Donald W. Luz, Christian Pedrini, Nicolás Fernandes, Éverton K. K. Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
topic_facet	Biología Biological control Entomopathogenic fungi Fungal UV tolerance Photoprotection Photoreactivation Vitamin B2
description	The aim of this study was to evaluate the effect of riboflavin supplementation of culture medium on conidial UV-A tolerance of M. acridum (Driver & Milner) (Hypocreales: Clavicipitaceae) and M. robertsii (Bischoff, Rehner & Humber) (Hypocreales: Clavicipitaceae). These fungi were produced on culture medium supplemented, or not supplemented, with riboflavin. Relative germination and expression patterns of some photoprotection-related genes were evaluated after irradiating with artificial UV-A, or with filtered solar radiation (> 320 nm; UV-A and visible radiation). M. acridum conidia harvested from riboflavin-supplemented culture medium demonstrated enhanced UV-A tolerance when irradiated with artificial UV-A. Nevertheless, relative germination of conidia of both species produced on riboflavin-supplemented medium and exposed to filtered solar radiation was significantly higher than those produced on medium not supplemented with riboflavin. Riboflavin increased the transcription of photolyases, laccases and polyketide synthase genes. However, each fungal species induced different genes patterns involved in DNA repair and photoprotection. The addition of riboflavin to the substrate used for mass production of Metarhizium spp. and the resulting enhancement of conidial tolerance to solar radiation may improve the effectiveness of these fungi in biological control programs.
format	Articulo Articulo
author	Huarte Bonnet, Carla Pereira Junior, Ronaldo Alves Santos da Paixão, Flávia Regina Braga, Gilberto U.L. Roberts, Donald W. Luz, Christian Pedrini, Nicolás Fernandes, Éverton K. K.
author_facet	Huarte Bonnet, Carla Pereira Junior, Ronaldo Alves Santos da Paixão, Flávia Regina Braga, Gilberto U.L. Roberts, Donald W. Luz, Christian Pedrini, Nicolás Fernandes, Éverton K. K.
author_sort	Huarte Bonnet, Carla
title	Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
title_short	Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
title_full	Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
title_fullStr	Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
title_full_unstemmed	Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes
title_sort	metarhizium robertsii and m. acridum conidia produced on riboflavin-supplemented medium have increased uv-a tolerance and upregulated photoprotection and photoreactivation genes
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/130954
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Metarhizium robertsii and M. acridum conidia produced on riboflavin-supplemented medium have increased UV-A tolerance and upregulated photoprotection and photoreactivation genes

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