Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases

Leptospirosis is a zoonotic disease of global distribution, which affects both animals and humans. Pathogenic leptospires, the bacteria that cause this disease, require iron for their growth, and these spirochetes probably use their hemolysins, such as the sphingomyelinases, as a way to obtain this...

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Detalles Bibliográficos
Autores principales: Carvalho, Eneas, Barbosa, Angela S., Gómez, Ricardo Martín, Oliveira, Maria L. S., Romero, Eliete C., Gonçales, Amane P., Morais, Zenaide M., Vasconcellos, Silvio A., Ho, Paulo L.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Biología
Recombinant Protein
Hemolytic Activity
Leptospirosis
Regular Secondary Structure
Pathogenic Leptospire
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/130948
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-130948
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Recombinant Protein
Hemolytic Activity
Leptospirosis
Regular Secondary Structure
Pathogenic Leptospire
spellingShingle Biología
Recombinant Protein
Hemolytic Activity
Leptospirosis
Regular Secondary Structure
Pathogenic Leptospire
Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Silvio A.
Ho, Paulo L.
Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
topic_facet Biología
Recombinant Protein
Hemolytic Activity
Leptospirosis
Regular Secondary Structure
Pathogenic Leptospire
description Leptospirosis is a zoonotic disease of global distribution, which affects both animals and humans. Pathogenic leptospires, the bacteria that cause this disease, require iron for their growth, and these spirochetes probably use their hemolysins, such as the sphingomyelinases, as a way to obtain this important nutrient from host red blood cells during infection. We expressed and purified the leptospiral sphingomyelinases Sph1, Sph2, Sph4, and SphH in a heterologous system. However, the recombinant proteins were not able to lyse sheep erythrocytes, despite having regular secondary structures. Transcripts for all sphingomyelinases tested were detected by RT-PCR analyses, but only Sph2 and SphH native proteins could be detected in Western blot assays using Leptospira whole extracts as well as in renal tubules of infected hamsters. Moreover, antibodies present in the serum of a human patient with laboratory-confirmed leptospirosis recognized Sph2, indicating that this sphingomyelinase is expressed and exposed to the immune system during infection in humans. However, in an animal challenge model, none of the sphingomyelinases tested conferred protection against leptospirosis.
format Articulo
Articulo
author Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Silvio A.
Ho, Paulo L.
author_facet Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Silvio A.
Ho, Paulo L.
author_sort Carvalho, Eneas
title Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
title_short Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
title_full Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
title_fullStr Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
title_full_unstemmed Evaluation of the Expression and Protective Potential of Leptospiral Sphingomyelinases
title_sort evaluation of the expression and protective potential of leptospiral sphingomyelinases
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/130948
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