Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells

We used transient expression assays to assess the function of the baculovirus <i>Spodoptera frugiperda</i> M nucleopolyhedrovirus (SfMNPV) homologs of <i>Autographa californica</i> MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, whi...

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Detalles Bibliográficos
Autores principales: Berretta, Marcelo Facundo, López, M. Gabriela, Taboga, Oscar, Sciocco de Cap, Alicia, Romanowski, Víctor
Formato: Articulo
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Exactas
Late expression factors
AcMNPV
SfMNPV
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/130915
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-130915
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Late expression factors
AcMNPV
SfMNPV
spellingShingle Ciencias Exactas
Late expression factors
AcMNPV
SfMNPV
Berretta, Marcelo Facundo
López, M. Gabriela
Taboga, Oscar
Sciocco de Cap, Alicia
Romanowski, Víctor
Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
topic_facet Ciencias Exactas
Late expression factors
AcMNPV
SfMNPV
description We used transient expression assays to assess the function of the baculovirus <i>Spodoptera frugiperda</i> M nucleopolyhedrovirus (SfMNPV) homologs of <i>Autographa californica</i> MNPV (AcMNPV) factors involved in late gene expression (lefs), in the Sf9 insect cell-line, which is permissive for both viruses. It is well-established that nineteen AcMNPV lefs support optimal levels of activity from a late promoter-reporter gene cassette in this assay. A subgroup of SfMNPV lefs predicted to function in transcription-specific events substituted the corresponding AcMNPV lefs very efficiently. When all SfMNPV lefs were assayed, including replication lefs, activity was low, but addition of two AcMNPV lefs not encoded in SfMNPV genome, resulted in augmented reporter activity. SfMNPV IE-1 was able to activate an early promoter cis-linked to an hr-derived element from SfMNPV but not from AcMNPV. However, the level of early promoter activation with SfMNPV IE-1 was lower compared to AcMNPV IE-1.
format Articulo
Articulo
author Berretta, Marcelo Facundo
López, M. Gabriela
Taboga, Oscar
Sciocco de Cap, Alicia
Romanowski, Víctor
author_facet Berretta, Marcelo Facundo
López, M. Gabriela
Taboga, Oscar
Sciocco de Cap, Alicia
Romanowski, Víctor
author_sort Berretta, Marcelo Facundo
title Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
title_short Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
title_full Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
title_fullStr Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
title_full_unstemmed Functional analysis of <i>Spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in Sf9 cells
title_sort functional analysis of <i>spodoptera frugiperda</i> nucleopolyhedrovirus late expression factors in sf9 cells
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/130915
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