Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography

This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the...

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Autores principales:	Suktham, Thirada, Jones, Andy, Acquaviva, Agustín, Dennis, Gary R., Shalliker, R. Andrew, Soliven, Arianne
Formato:	Articulo Preprint
Lenguaje:	Inglés
Publicado:	2020
Materias:	Ciencias Exactas Química total antioxidant capacity reaction flow chromatography post column derivatisation selective detection cupric reducing antioxidant capacity
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/128797
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-128797
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Química total antioxidant capacity reaction flow chromatography post column derivatisation selective detection cupric reducing antioxidant capacity
spellingShingle	Ciencias Exactas Química total antioxidant capacity reaction flow chromatography post column derivatisation selective detection cupric reducing antioxidant capacity Suktham, Thirada Jones, Andy Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
topic_facet	Ciencias Exactas Química total antioxidant capacity reaction flow chromatography post column derivatisation selective detection cupric reducing antioxidant capacity
description	This study is based upon a recently established method for quantification of the antioxidant capacity of natural samples via a HPLC separation and a hyphenated selective detection (post-column derivatization with cupric reducing antioxidant capacity reagent) technique. This protocol demonstrated the main improvements to transform the quantitative protocol into a high-speed qualitative automated assay to screen samples for their potential total antioxidant capacity, typically performed via manual mixing of the sample and derivatisation and measured on a 96 well plate reader/bench top UV–Vis spectrophotometer. This approach with automated mixing is a more informative alternative for total antioxidant capacity as the antioxidant peaks are profiled for each sample within four minutes. This antioxidant profile may be used for routine analysis of raw materials and/or a guide for targeted approaches for structure elucidation for laboratories interested in early drug discovery, natural product research and the search of alternative antioxidant additives in consumer goods/therapeutics. This technique could also be used to monitor the stability, alteration or adulteration of manufactured goods containing antioxidants.
format	Articulo Preprint
author	Suktham, Thirada Jones, Andy Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne
author_facet	Suktham, Thirada Jones, Andy Acquaviva, Agustín Dennis, Gary R. Shalliker, R. Andrew Soliven, Arianne
author_sort	Suktham, Thirada
title	Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_short	Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_full	Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_fullStr	Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_full_unstemmed	Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
title_sort	better than bench top : high speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/128797
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bdutipo_str	Repositorios
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Better than bench top : High speed antioxidant screening via the cupric reducing antioxidant capacity reagent and reaction flow chromatography

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