Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus
Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is curre...
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Autores principales: | , , , , |
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Formato: | Articulo Preprint |
Lenguaje: | Inglés |
Publicado: |
2016
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Materias: | |
Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/128754 |
Aporte de: |
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I19-R120-10915-128754 |
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record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Biología Ciencias Médicas Stress Ghrelin Orexigenic γ-aminobutyric acid (GABA) Neuropeptide Y (NPY) |
spellingShingle |
Biología Ciencias Médicas Stress Ghrelin Orexigenic γ-aminobutyric acid (GABA) Neuropeptide Y (NPY) Cabral, Agustina Soledad Portiansky, Enrique Leo Sánchez Jaramillo, Edith Zigman, Jeffrey M. Perelló, Mario Carlos Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
topic_facet |
Biología Ciencias Médicas Stress Ghrelin Orexigenic γ-aminobutyric acid (GABA) Neuropeptide Y (NPY) |
description |
Previous work has established that the hormone ghrelin engages the hypothalamic-pituitary-adrenal neuroendocrine axis via activation of corticotropin-releasing factor (CRF) neurons of the hypothalamic paraventricular nucleus (PVN). The neuronal circuitry that mediates this effect of ghrelin is currently unknown. Here, we show that ghrelin-induced activation of PVN CRF neurons involved inhibition of γ-aminobutyric acid (GABA) inputs, likely via ghrelin binding sites that were localized at GABAergic terminals within the PVN. While ghrelin activated PVN CRF neurons in the presence of neuropeptide Y (NPY) receptor antagonists or in arcuate nucleus (ARC)-ablated mice, it failed to do it so in mice with ghrelin receptor expression limited to ARC agouti gene related protein (AgRP)/NPY neurons. These data support the notion that ghrelin activates PVN CRF neurons via inhibition of local GABAergic tone, in an ARC-independent manner. Furthermore, these data suggest that the neuronal circuits mediating ghrelin's orexigenic action vs. its role as a stress signal are anatomically dissociated. |
format |
Articulo Preprint |
author |
Cabral, Agustina Soledad Portiansky, Enrique Leo Sánchez Jaramillo, Edith Zigman, Jeffrey M. Perelló, Mario Carlos |
author_facet |
Cabral, Agustina Soledad Portiansky, Enrique Leo Sánchez Jaramillo, Edith Zigman, Jeffrey M. Perelló, Mario Carlos |
author_sort |
Cabral, Agustina Soledad |
title |
Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
title_short |
Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
title_full |
Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
title_fullStr |
Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
title_full_unstemmed |
Ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
title_sort |
ghrelin activates hypophysiotropic corticotropin-releasing factor neurons independently of the arcuate nucleus |
publishDate |
2016 |
url |
http://sedici.unlp.edu.ar/handle/10915/128754 |
work_keys_str_mv |
AT cabralagustinasoledad ghrelinactivateshypophysiotropiccorticotropinreleasingfactorneuronsindependentlyofthearcuatenucleus AT portianskyenriqueleo ghrelinactivateshypophysiotropiccorticotropinreleasingfactorneuronsindependentlyofthearcuatenucleus AT sanchezjaramilloedith ghrelinactivateshypophysiotropiccorticotropinreleasingfactorneuronsindependentlyofthearcuatenucleus AT zigmanjeffreym ghrelinactivateshypophysiotropiccorticotropinreleasingfactorneuronsindependentlyofthearcuatenucleus AT perellomariocarlos ghrelinactivateshypophysiotropiccorticotropinreleasingfactorneuronsindependentlyofthearcuatenucleus |
bdutipo_str |
Repositorios |
_version_ |
1764820452813832192 |