DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeli...
| Autores principales: | , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2014
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/128275 |
| Aporte de: |
| id |
I19-R120-10915-128275 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling |
| spellingShingle |
Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling Silva, Adriana L. Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| topic_facet |
Bioquímica Allergy gene therapy Thymulin DNA nanoparticles Lung remodeling |
| description |
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK30PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics. |
| format |
Articulo Articulo |
| author |
Silva, Adriana L. Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. |
| author_facet |
Silva, Adriana L. Martini, Sabrina V. Abreu, Soraia C. Samary, Cynthia S. Diaz, Bruno L. Fernezlian, Sandra Sá, Vanessa Karen de Capelozzi, Vera Luiza Boylan, Nicholas J. Goya, Rodolfo Gustavo Suk, Jung Soo Rocco, Patricia R. M. Hanes, Justin Morales, Marcelo M. |
| author_sort |
Silva, Adriana L. |
| title |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_short |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_full |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_fullStr |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_full_unstemmed |
DNA nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| title_sort |
dna nanoparticle-mediated thymulin gene therapy prevents airway remodeling in experimental allergic asthma |
| publishDate |
2014 |
| url |
http://sedici.unlp.edu.ar/handle/10915/128275 |
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