Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats

Progressive dysfunction of hypothalamic tu-beroinfundibular dopaminergic (TIDA) neurons during nor-mal aging is associated in the female rat with chronic hyper-prolactinemia. We assessed the effectiveness of glial cellline-derived neurotrophic factor (GDNF) gene therapy to re-store TIDA neuron funct...

Descripción completa

Detalles Bibliográficos
Autores principales:	Morel, Gustavo Ramón, Sosa, Yolanda Elena, Bellini, María José, Carri, Néstor Gabriel, Rodríguez, Silvia Susana, Bohn, Martha C., Goya, Rodolfo Gustavo
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Biología Aging DA neurodegeneration TIDA neurons chronic hyperprolactinemia GDNF gene therapy
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/128193
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-128193
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Aging DA neurodegeneration TIDA neurons chronic hyperprolactinemia GDNF gene therapy
spellingShingle	Biología Aging DA neurodegeneration TIDA neurons chronic hyperprolactinemia GDNF gene therapy Morel, Gustavo Ramón Sosa, Yolanda Elena Bellini, María José Carri, Néstor Gabriel Rodríguez, Silvia Susana Bohn, Martha C. Goya, Rodolfo Gustavo Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
topic_facet	Biología Aging DA neurodegeneration TIDA neurons chronic hyperprolactinemia GDNF gene therapy
description	Progressive dysfunction of hypothalamic tu-beroinfundibular dopaminergic (TIDA) neurons during nor-mal aging is associated in the female rat with chronic hyper-prolactinemia. We assessed the effectiveness of glial cellline-derived neurotrophic factor (GDNF) gene therapy to re-store TIDA neuron function in senile female rats and reversetheir chronic hyperprolactinemia. Young (2.5 months) andsenile (29 months) rats received a bilateral intrahypothalamicinjection (1010pfu) of either an adenoviral vector expressingthe gene for β-galactosidase; (Y-βgal and S-βgal, respectively) or a vector expressing rat GDNF (Y-GDNF and S-GDNF,respectively). Transgenic GDNF levels in supernatants ofGDNF adenovector-transduced N2a neuronal cell cultureswere 25±4 ng/ml, as determined by bioassay. In the rats,serum prolactin (PRL) was measured at regular intervals. Onday 17 animals were sacrificed and neuronal nuclear antigen(NeuN) and tyrosine hydroxylase (TH) immunoreactive cellscounted in the arcuate–periventricular hypothalamic region.The S-GDNF but not the S-βgal rats, showed a significantreduction in body weight. The chronic hyperprolactinemia ofthe senile females was significantly ameliorated in the S-GDNF rats (P<0.05) but not in the S-βgal rats. Neither age norGDNF induced significant changes in the number of NeuNand TH neurons. We conclude that transgenic GDNF amelio-rates chronic hyperprolactinemia in aging female rats, prob-ably by restoring TIDA neuron function.
format	Articulo Articulo
author	Morel, Gustavo Ramón Sosa, Yolanda Elena Bellini, María José Carri, Néstor Gabriel Rodríguez, Silvia Susana Bohn, Martha C. Goya, Rodolfo Gustavo
author_facet	Morel, Gustavo Ramón Sosa, Yolanda Elena Bellini, María José Carri, Néstor Gabriel Rodríguez, Silvia Susana Bohn, Martha C. Goya, Rodolfo Gustavo
author_sort	Morel, Gustavo Ramón
title	Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
title_short	Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
title_full	Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
title_fullStr	Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
title_full_unstemmed	Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
title_sort	glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/128193
work_keys_str_mv	AT morelgustavoramon glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT sosayolandaelena glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT bellinimariajose glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT carrinestorgabriel glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT rodriguezsilviasusana glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT bohnmarthac glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats AT goyarodolfogustavo glialcelllinederivedneurotrophicfactorgenetherapyameliorateschronichyperprolactinemiainsenilerats
bdutipo_str	Repositorios
_version_	1764820452066197504

Glial cell line-derived neurotrophic factor gene therapy ameliorates chronic hyperprolactinemia in senile rats

Ejemplares similares