Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions

Polyamine Phosphate Nanoparticles (PANs) have great potential for the delivery of large therapeutics, such as plasmids and/or siRNAs. The formation of PANs by complexation of Poly(allylamine hydrochloride) (PAH) and phosphate ions from Phosphate Buffer (PB) was studied here, and how it is affected b...

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Autores principales: Andreozzi, Patrizia, Ricci, Caterina, Martínez Porcel, Joaquín, Moretti, Paolo, Di Silvio, Desirè, Amenitsch, Heinz, Ortore, Maria Grazia, Moya, Sergio
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Química
Polyamine phosphate interactions
Nanoparticles nucleation
Self-assembled nanoparticles
SAXS
DLS
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127919
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127919
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Polyamine phosphate interactions
Nanoparticles nucleation
Self-assembled nanoparticles
SAXS
DLS
spellingShingle Química
Polyamine phosphate interactions
Nanoparticles nucleation
Self-assembled nanoparticles
SAXS
DLS
Andreozzi, Patrizia
Ricci, Caterina
Martínez Porcel, Joaquín
Moretti, Paolo
Di Silvio, Desirè
Amenitsch, Heinz
Ortore, Maria Grazia
Moya, Sergio
Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
topic_facet Química
Polyamine phosphate interactions
Nanoparticles nucleation
Self-assembled nanoparticles
SAXS
DLS
description Polyamine Phosphate Nanoparticles (PANs) have great potential for the delivery of large therapeutics, such as plasmids and/or siRNAs. The formation of PANs by complexation of Poly(allylamine hydrochloride) (PAH) and phosphate ions from Phosphate Buffer (PB) was studied here, and how it is affected by the presence of phosphate ions from PB and ionic strength. From Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS) the critical PB concentration for PANs formation was determined. Below this critical point, Small Angle X-ray Scattering (SAXS) studies revealed that small PAH-phosphate aggregates coexist with not complexed or weakly complexed polymer chains in solution and that the presence of the phosphate ions increases the Kuhn length of the polymer chains until that only spherical aggregates are present in solution. TEM, DLS and SAXS showed the increase of PANs size with ionic strength up to 250 mM NaCl. At higher NaCl concentrations, PANs disassemble into smaller aggregates. Isothermal Titration Calorimetry (ITC) showed that PAN formation is an exothermic process and the association of phosphates below the critical PB concentration is entropically controlled.
format Articulo
Preprint
author Andreozzi, Patrizia
Ricci, Caterina
Martínez Porcel, Joaquín
Moretti, Paolo
Di Silvio, Desirè
Amenitsch, Heinz
Ortore, Maria Grazia
Moya, Sergio
author_facet Andreozzi, Patrizia
Ricci, Caterina
Martínez Porcel, Joaquín
Moretti, Paolo
Di Silvio, Desirè
Amenitsch, Heinz
Ortore, Maria Grazia
Moya, Sergio
author_sort Andreozzi, Patrizia
title Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
title_short Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
title_full Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
title_fullStr Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
title_full_unstemmed Mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
title_sort mechanistic study of the nucleation and conformational changes of polyamines in presence of phosphate ions
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/127919
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