DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction

Cuticle tanning occurs in insects immediately after hatching or molting. During this process, the cuticle becomes dark and rigid due to melanin deposition and protein crosslinking. In insects, different from mammals, melanin is synthesized mainly from dopamine, which is produced from DOPA by the enz...

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Detalles Bibliográficos
Autores principales: Sterkel, Marcos, Ons, Sheila, Oliveira, Pedro L.
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2019
Materias:
Bioquímica
Aematophagous arthropods
Aromatic amino acid decarboxylases
Melanin synthesis
Tyrosine metabolism
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127906
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127906
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Aematophagous arthropods
Aromatic amino acid decarboxylases
Melanin synthesis
Tyrosine metabolism
spellingShingle Bioquímica
Aematophagous arthropods
Aromatic amino acid decarboxylases
Melanin synthesis
Tyrosine metabolism
Sterkel, Marcos
Ons, Sheila
Oliveira, Pedro L.
DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
topic_facet Bioquímica
Aematophagous arthropods
Aromatic amino acid decarboxylases
Melanin synthesis
Tyrosine metabolism
description Cuticle tanning occurs in insects immediately after hatching or molting. During this process, the cuticle becomes dark and rigid due to melanin deposition and protein crosslinking. In insects, different from mammals, melanin is synthesized mainly from dopamine, which is produced from DOPA by the enzyme DOPA decarboxylase. In this work, we report that the silencing of the RpAadc-2 gene, which encodes the putative Rhodnius prolixus DOPA decarboxylase enzyme, resulted in a reduction in nymph survival, with a high percentage of treated insects dying during the ecdysis process or in the expected ecdysis period. Those treated insects that could complete ecdysis presented a decrease in cuticle pigmentation and hardness after molting. In adult females, the knockdown of AADC-2 resulted in a reduction in the hatching of eggs; the nymphs that managed to hatch failed to tan the cuticle and were unable to feed. Despite the failure in cuticle tanning, knockdown of the AADC-2 did not increase the susceptibility to topically applied deltamethrin, a pyrethroid insecticide. Additionally, our results showed that the melanin synthesis pathway did not play a major role in the detoxification of the excess (potentially toxic) tyrosine from the diet, an essential trait for hematophagous arthropod survival after a blood meal.
format Articulo
Preprint
author Sterkel, Marcos
Ons, Sheila
Oliveira, Pedro L.
author_facet Sterkel, Marcos
Ons, Sheila
Oliveira, Pedro L.
author_sort Sterkel, Marcos
title DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
title_short DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
title_full DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
title_fullStr DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
title_full_unstemmed DOPA decarboxylase is essential for cuticle tanning in Rhodnius prolixus (Hemiptera: Reduviidae), affecting ecdysis, survival and reproduction
title_sort dopa decarboxylase is essential for cuticle tanning in rhodnius prolixus (hemiptera: reduviidae), affecting ecdysis, survival and reproduction
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/127906
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AT oliveirapedrol dopadecarboxylaseisessentialforcuticletanninginrhodniusprolixushemipterareduviidaeaffectingecdysissurvivalandreproduction
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