Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition

Rationale: Proteins from the egg perivitelline fluid (PVF) are assumed to play critical roles in embryonic development, but for many groups of animals their identities remain unknown. Identifying egg PVF proteins is a critical step towards understanding their functions including their roles in evolu...

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Detalles Bibliográficos
Autores principales: Ip, Jack C.H., Mu, Huawei, Zhang, Yanjie, Heras, Horacio, Qiu, Jian-Wen
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Apple snail
Proteomics
Egg perivitelline
Terrestrial eggs
Positive selection
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127372
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127372
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Apple snail
Proteomics
Egg perivitelline
Terrestrial eggs
Positive selection
spellingShingle Biología
Apple snail
Proteomics
Egg perivitelline
Terrestrial eggs
Positive selection
Ip, Jack C.H.
Mu, Huawei
Zhang, Yanjie
Heras, Horacio
Qiu, Jian-Wen
Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
topic_facet Biología
Apple snail
Proteomics
Egg perivitelline
Terrestrial eggs
Positive selection
description Rationale: Proteins from the egg perivitelline fluid (PVF) are assumed to play critical roles in embryonic development, but for many groups of animals their identities remain unknown. Identifying egg PVF proteins is a critical step towards understanding their functions including their roles in evolutionary transition in habitats. Methods: We applied proteomic and transcriptomic analysis to investigate the PVF proteome of the eggs of Pomacea diffusa, an aerial ovipositing freshwater snail in the family Ampullariidae. The PVF proteins were separated with the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method, and proteomic analysis was conducted using an LTQ Velos ion trap mass spectrometer coupled with liquid chromatography. Comparison of PVF proteomes and evolution analyses was performed between P. diffusa and other ampullariids. Results In total, 32 egg PVF proteins were identified from P. diffusa. They were categorized as PV1-like subunits, immune-responsive proteins, protein degradation, signaling and binding, transcription and translation, metabolism, oxidation-reduction and proteins with unknown function. Interestingly, the proteome includes a calcium-binding protein important in forming the hard eggshell that enabled the terrestrial transition. However, it does not include PV2, a neurotoxic protein that was assumed to be present in all Pomacea species. Conclusions: The PVF proteome data from P. diffusa can help us better understand the roles that reproductive proteins played during the transition from underwater to terrestrial egg deposition. Moreover, they could be useful in comparative studies of the terrestrialization in several groups of animals that occurred independently during their evolution.
format Articulo
Preprint
author Ip, Jack C.H.
Mu, Huawei
Zhang, Yanjie
Heras, Horacio
Qiu, Jian-Wen
author_facet Ip, Jack C.H.
Mu, Huawei
Zhang, Yanjie
Heras, Horacio
Qiu, Jian-Wen
author_sort Ip, Jack C.H.
title Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
title_short Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
title_full Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
title_fullStr Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
title_full_unstemmed Egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
title_sort egg perivitelline fluid proteome of a freshwater snail: insight into the transition from aquatic to terrestrial egg deposition
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/127372
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