nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics

A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance...

Descripción completa

Detalles Bibliográficos
Autores principales: Collavino, Mónica Mariana, Tripp, H. James, Frank, Ildiko E., Vidoz, María Laura, Calderoli, Priscila Anabel, Donato, Mariano Humberto, Zehr, Jonathan P., Aguilar, Orlando Mario
Formato: Articulo
Lenguaje:Inglés
Publicado: 2014
Materias:
Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127286
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127286
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
spellingShingle Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
topic_facet Ciencias Naturales
Biología
nifH pyrosequencing
soil chemistry
phylogenetic analysis
Bacteria
description A dataset of 87 020 <i>nifH</i> reads and 16 782 unique <i>nifH</i> protein sequences obtained over 2 years from four locations across a gradient of agricultural soil types in Argentina were analysed to provide a detailed and comprehensive picture of the diversity, abundance and responses of the N₂-fixing community in relation to differences in soil chemistry and agricultural practices. Phylogenetic analysis revealed an expected high proportion of <i>Alphaproteobacteria</i>, <i>Betaproteobacteria</i> and <i>Deltaproteobacteria</i>, mainly relatives to <i>Bradyrhizobium</i> and <i>Methylosinus/Methylocystis</i>, but a surprising paucity of <i>Gammaproteobacteria</i>. Analysis of variance and stepwise regression modelling suggested location and treatment-specific influences of soil type on diazotrophic community composition and organic carbon concentrations on <i>nifH</i> diversity. <i>nifH</i> gene abundance, determined by quantitative real-time polymerase chain reaction, was higher in agricultural soils than in non-agricultural soils, and was influenced by soil chemistry under intensive crop rotation but not under monoculture. At some locations, sustainable increased crop yields might be possible through the management of soil chemistry to improve the abundance and diversity of N₂-fixing bacteria.
format Articulo
Articulo
author Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author_facet Collavino, Mónica Mariana
Tripp, H. James
Frank, Ildiko E.
Vidoz, María Laura
Calderoli, Priscila Anabel
Donato, Mariano Humberto
Zehr, Jonathan P.
Aguilar, Orlando Mario
author_sort Collavino, Mónica Mariana
title nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_short nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_full nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_fullStr nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_full_unstemmed nifH pyrosequencing reveals the potential for location-specific soil chemistry to influence N₂-fixing community dynamics
title_sort nifh pyrosequencing reveals the potential for location-specific soil chemistry to influence n₂-fixing community dynamics
publishDate 2014
url http://sedici.unlp.edu.ar/handle/10915/127286
work_keys_str_mv AT collavinomonicamariana nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT tripphjames nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT frankildikoe nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT vidozmarialaura nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT calderolipriscilaanabel nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT donatomarianohumberto nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT zehrjonathanp nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
AT aguilarorlandomario nifhpyrosequencingrevealsthepotentialforlocationspecificsoilchemistrytoinfluencen2fixingcommunitydynamics
bdutipo_str Repositorios
_version_ 1764820451545055234

Ejemplares similares