Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model
Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve t...
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| Autores principales: | , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2012
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/127095 |
| Aporte de: |
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I19-R120-10915-127095 |
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dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Médicas Acute tubular necrosis Thymoglobulin Brain death MCP-1 IL-10 |
| spellingShingle |
Ciencias Médicas Acute tubular necrosis Thymoglobulin Brain death MCP-1 IL-10 Cicora, Federico Stringa, Pablo Luis Guerrieri, D. Roberti, J. Ambrosi, N. Toniolo, F. Cicora, P. Palti, G. Vásquez, D. Raimondi, Jorge Clemente Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| topic_facet |
Ciencias Médicas Acute tubular necrosis Thymoglobulin Brain death MCP-1 IL-10 |
| description |
Brain death (BD), a non-immunological factor of renal injury, triggers an inflammatory process causing pathological signs of cell death in the kidney, such as necrosis and apoptosis. Kidneys from brain dead donors show lower success rates than kidneys from living donors and one strategy to improve transplantation outcome is to precondition the donors. For the first time, anti-rat thymoglobulin (rATG) was administered in an experimental brain death animal model to evaluate if it could ameliorate histopathological damage and improve organ function. Animals were divided into three groups: V (n = 5) ventilated for 2 h; BD (n = 5) brain death and ventilated for 2 h; and BD+rATG (n = 5) brain death, ventilated for 2 h, rATG was administered during brain death (10 mg/kg). We observed lower creatinine levels in treatment groups (means): V, 0·88 ± 0·22 mg/dl; BD, 1·37 ± 0·07 mg/dl; and BD+rATG, 0·64 ± 0·02 mg/dl (BD versus BD+rATG, P < 0·001). In the BD group there appeared to be a marked increase of ATN, whereas ATN was decreased significantly in the rATG group (V, 2·25 ± 0·5 versus BD, 4·75 ± 0·5, P < 0·01; BD+rATG, 2·75 ± 0·5 versus BD 4·75 ± 0·5 P < 0·01). Gene expression was evaluated with reverse transcription–polymerase chain reaction; tumour necrosis factor (TNF)-α, interleukin (IL)-6, C3, CD86 showed no significant difference between groups. Increased IL-10 and decreased CCL2 in BD+rATG compared to BD (both cases P < 0·01). Myeloperoxidase was increased significantly after the brain death setting (V: 32 ± 7·5 versus BD: 129 ± 18). Findings suggest that rATG administered to potential donors may ameliorate renal damage caused by BD. These findings could contribute in the search for specific cytoprotective interventions to improve the quality and viability of transplanted organs. |
| format |
Articulo Articulo |
| author |
Cicora, Federico Stringa, Pablo Luis Guerrieri, D. Roberti, J. Ambrosi, N. Toniolo, F. Cicora, P. Palti, G. Vásquez, D. Raimondi, Jorge Clemente |
| author_facet |
Cicora, Federico Stringa, Pablo Luis Guerrieri, D. Roberti, J. Ambrosi, N. Toniolo, F. Cicora, P. Palti, G. Vásquez, D. Raimondi, Jorge Clemente |
| author_sort |
Cicora, Federico |
| title |
Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| title_short |
Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| title_full |
Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| title_fullStr |
Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| title_full_unstemmed |
Amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| title_sort |
amelioration of renal damage by administration of anti-thymocyte globulin to potential donors in a brain death rat model |
| publishDate |
2012 |
| url |
http://sedici.unlp.edu.ar/handle/10915/127095 |
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Repositorios |
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