Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs

Background Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in the diet could result in increased lipid peroxidation and damage to proteins, lipids and DNA. We evaluated the effect of dietar...

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Detalles Bibliográficos
Autores principales: Pellegrino, Francisco Javier, Risso, Analía Lorena, Corrada, Yanina Alejandra, Gambaro, Rocío Celeste, Seoane, Analía Isabel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Veterinarias
Internal medicine
Endocrinology
Cholesterol
Lipid profile
Malondialdehyde
Triglyceride
Fish oil
Lipoprotein
Lipid peroxidation
Unsaturated fatty acid
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/127085
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-127085
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Internal medicine
Endocrinology
Cholesterol
Lipid profile
Malondialdehyde
Triglyceride
Fish oil
Lipoprotein
Lipid peroxidation
Unsaturated fatty acid
spellingShingle Ciencias Veterinarias
Internal medicine
Endocrinology
Cholesterol
Lipid profile
Malondialdehyde
Triglyceride
Fish oil
Lipoprotein
Lipid peroxidation
Unsaturated fatty acid
Pellegrino, Francisco Javier
Risso, Analía Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analía Isabel
Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
topic_facet Ciencias Veterinarias
Internal medicine
Endocrinology
Cholesterol
Lipid profile
Malondialdehyde
Triglyceride
Fish oil
Lipoprotein
Lipid peroxidation
Unsaturated fatty acid
description Background Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in the diet could result in increased lipid peroxidation and damage to proteins, lipids and DNA. We evaluated the effect of dietary FO supplementation on DNA damage in peripheral blood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods Healthy male dogs (n = 9) were randomly assigned to one of two diets during 90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), the same food supplemented with 1000 mg FO. Blood samples were collected on days -1, 30, 60 and 90. DNA damage was assessed with the comet assay, and the damage index was obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipid peroxidation. Lipid profile determination included serum triglyceride, cholesterol, low-density lipoprotein and high-density lipoprotein levels (HDL). Results Damage index values (arbitrary units) were lower in FOG on day 30 (CG, 13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ± 2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and 90 (treatment × time interaction, p < 0.05). Conclusion These findings suggest that dietary FO supplementation did not induce DNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.
format Articulo
Articulo
author Pellegrino, Francisco Javier
Risso, Analía Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analía Isabel
author_facet Pellegrino, Francisco Javier
Risso, Analía Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analía Isabel
author_sort Pellegrino, Francisco Javier
title Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_short Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_full Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_fullStr Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_full_unstemmed Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_sort influence of dietary fish oil supplementation on dna damage in peripheral blood lymphocytes of nine healthy dogs
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/127085
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