Characterizing monoclonal antibody epitopes by filtered gene fragment phage display
In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to se...
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Autores principales: | , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2005
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/126484 |
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I19-R120-10915-126484 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Bioquímica Phage display Epitope mapping β-lactamase Transglutaminase |
spellingShingle |
Bioquímica Phage display Epitope mapping β-lactamase Transglutaminase Di Niro, Roberto Ferrara, Fortunato Not, Tarcisio Bradbury, Andrew Chirdo, Fernando Gabriel Marzari, Roberto Sblattero, Daniele Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
topic_facet |
Bioquímica Phage display Epitope mapping β-lactamase Transglutaminase |
description |
In the present paper, we describe a novel approach to map monoclonal antibody epitopes, using three new monoclonal antibodies that recognize h-TG2 (human transglutaminase 2) as an example. The target gene was fragmented and cloned upstream of an antibiotic-resistance gene, in the vector pPAO2, to select for in-frame polypeptides. After removal of the antibiotic-resistance gene by Cre/Lox recombination, an antigen fragment phage display library was created and selected against specific monoclonal antibodies. Using the h-TG2 fragment library, we were able to identify epitopes. This technique can also be broadly applied to the study of protein-protein interactions. |
format |
Articulo Articulo |
author |
Di Niro, Roberto Ferrara, Fortunato Not, Tarcisio Bradbury, Andrew Chirdo, Fernando Gabriel Marzari, Roberto Sblattero, Daniele |
author_facet |
Di Niro, Roberto Ferrara, Fortunato Not, Tarcisio Bradbury, Andrew Chirdo, Fernando Gabriel Marzari, Roberto Sblattero, Daniele |
author_sort |
Di Niro, Roberto |
title |
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
title_short |
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
title_full |
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
title_fullStr |
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
title_full_unstemmed |
Characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
title_sort |
characterizing monoclonal antibody epitopes by filtered gene fragment phage display |
publishDate |
2005 |
url |
http://sedici.unlp.edu.ar/handle/10915/126484 |
work_keys_str_mv |
AT diniroroberto characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT ferrarafortunato characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT nottarcisio characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT bradburyandrew characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT chirdofernandogabriel characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT marzariroberto characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay AT sblatterodaniele characterizingmonoclonalantibodyepitopesbyfilteredgenefragmentphagedisplay |
bdutipo_str |
Repositorios |
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1764820450764914689 |