A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs

The acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its pr...

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Detalles Bibliográficos
Autores principales: Brola, Tabata Romina, Dreon, Marcos Sebastián, Qiu, Jian-Wen, Heras, Horacio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/126164
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-126164
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
spellingShingle Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
Brola, Tabata Romina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
topic_facet Biología
Ampullariidae
Egg defense
Gastropods
Glycan array
Lectin activity
Protein structure
description The acquisition of egg protection is vital for species survival. Poisonous eggs from Pomacea apple snails have defensive macromolecules for protection. Here we isolated and characterized a novel lectin called PdPV1 that is massively accumulated in the eggs of Pomacea diffusa and seems part of its protective cocktail. The native protein, an oligomer of ca. 256 kDa, has high structural stability, withstanding 15 min boiling and denaturing by sodium dodecyl sulphate. It resists in vitro proteinase digestion and displays structural stability between pH 2.0-12.0 and up to 85 °C. These properties, as well as its subunit sequences, glycosylation pattern, presence of carotenoids, size, and global shape resemble those of its orthologs from other Pomacea. Further, like members of the canaliculata clade, PdPV1 is recovered unchanged in faeces of mice ingesting it, supporting an antinutritive defensive function. PdPV1 also displays a strong hemagglutinating activity specifically recognizing selected ganglioside motifs with high affinity. This activity is only shared with PsSC, a perivitelline from the same clade (bridgesii clade). As a whole, these results indicate that species in the genus Pomacea have diversified their eggs defences: Those from the bridgesii clade are protected mostly by non-digestible lectins that lower the nutritional value of eggs, in contrast with protection by neurotoxins of other Pomacea clades, indicating apple snail egg defensive strategies are clade-specific. The harsh gastrointestinal environment of predators would have favoured their appearance, extending by convergent evolution the presence of plant-like highly stable lectins, a strategy not reported in other animals.
format Articulo
Articulo
author Brola, Tabata Romina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
author_facet Brola, Tabata Romina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Heras, Horacio
author_sort Brola, Tabata Romina
title A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_short A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_full A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_fullStr A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_full_unstemmed A highly stable, nondigestible lectin from Pomacea diffusa unveils clade-related protection systems in apple snail eggs
title_sort highly stable, nondigestible lectin from pomacea diffusa unveils clade-related protection systems in apple snail eggs
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/126164
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