First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA

It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating...

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Detalles Bibliográficos
Autores principales: Beltrame, María Ornela, Pruzzo, César Iván, Sanabria, Rodrigo Eduardo Fabrizio, Pérez, Alberto E., Mora, Matías Sebastián
Formato: Articulo
Lenguaje:Inglés
Publicado: 2019
Materias:
Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125996
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125996
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
spellingShingle Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
Beltrame, María Ornela
Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
topic_facet Ciencias Veterinarias
aDNA
DEER
Fasciola hepatica
Palaeoparasitology
description It is generally assumed that the digenean human liver fluke, Fasciola hepatica, gained entry to South America during the 15th century upon arrival of Europeans and their livestock. Nonetheless in Patagonia, Argentina, digenean eggs similar to F. hepatica have been observed in deer coprolites dating back to 2300 years B.P. The main objective of our present study was to identify and characterize these eggs using an ancient DNA (aDNA) study. Eggs were isolated and used for aDNA extraction, amplification and sequencing of partial regions from the cytochrome c oxidase subunit 1 and the nicotinamide adenine dinucleotide dehydrogenase subunit 1 mitochondrial genes. Also, phylogenetic trees were constructed using Bayesian and maximum likelihood. Our results confirm the presence of F. hepatica in South America from at least 2300 years B.P. This is the first report and the first aDNA study of this trematode in South America prior to the arrival of the European cattle in the 15th century. The present work contributes to the study of phylogenetic and palaeobiogeographical aspects of F. hepatica and its settlement across America.
format Articulo
Articulo
author Beltrame, María Ornela
Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
author_facet Beltrame, María Ornela
Pruzzo, César Iván
Sanabria, Rodrigo Eduardo Fabrizio
Pérez, Alberto E.
Mora, Matías Sebastián
author_sort Beltrame, María Ornela
title First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_short First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_fullStr First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_full_unstemmed First report of pre-Hispanic Fasciola hepatica from South America revealed by ancient DNA
title_sort first report of pre-hispanic fasciola hepatica from south america revealed by ancient dna
publishDate 2019
url http://sedici.unlp.edu.ar/handle/10915/125996
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