Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle

Toxoplasma gondii is an apicomplexan intracellular protozoan parasite responsible for toxoplasmosis, a disease with considerable medical and economic impact worldwide. Toxoplasma gondii cells never lose the nuclear envelope and their chromosomes do not condense. Here, we tested the murine monoclonal...

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Autores principales: Vanagas, Laura, Dalmasso, Maria Carolina, Dubremetz, Jean François, Portiansky, Enrique Leo, Olins, Donald E., Angel, Sergio O.
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2013
Materias:
Ciencias Veterinarias
Toxoplasma gondii
Epichromatin
Nuclear envelope
Cell cycle
Replication
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125918
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125918
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Toxoplasma gondii
Epichromatin
Nuclear envelope
Cell cycle
Replication
spellingShingle Ciencias Veterinarias
Toxoplasma gondii
Epichromatin
Nuclear envelope
Cell cycle
Replication
Vanagas, Laura
Dalmasso, Maria Carolina
Dubremetz, Jean François
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio O.
Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
topic_facet Ciencias Veterinarias
Toxoplasma gondii
Epichromatin
Nuclear envelope
Cell cycle
Replication
description Toxoplasma gondii is an apicomplexan intracellular protozoan parasite responsible for toxoplasmosis, a disease with considerable medical and economic impact worldwide. Toxoplasma gondii cells never lose the nuclear envelope and their chromosomes do not condense. Here, we tested the murine monoclonal antibody PL2-6, which labels epichromatin (a conformational chromatin epitope based on histones H2A and H2B complexed with DNA), in T. gondii cultured in human fibroblasts. This epitope is present at the exterior chromatin surface of interphase nuclei and on the periphery of mitotic chromosomes in higher eukaryotes. PL2-6 reacted with T. gondii H2A and H2B histones in Western blot (WB) assays. In addition, the antibody reacted with the nuclear fraction of tachyzoites, as a single band coincident with H2B histone. In the T. gondii tachyzoite stage, PL2-6 also had peripheral nuclear localization, as observed by epifluorescence/confocal microscopy and immunoelectron microscopy. Confocal analysis showed that epichromatin is slightly polarized to one face of the parasite exterior chromatin surface. In replicating tachyzoites, PL2-6 also labels the exterior chromatin surface, covering the face of both segregating nuclei, facing the plasma membrane of the mother cell. The possible role of epichromatin in T. gondii is discussed.
format Articulo
Preprint
author Vanagas, Laura
Dalmasso, Maria Carolina
Dubremetz, Jean François
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio O.
author_facet Vanagas, Laura
Dalmasso, Maria Carolina
Dubremetz, Jean François
Portiansky, Enrique Leo
Olins, Donald E.
Angel, Sergio O.
author_sort Vanagas, Laura
title Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_short Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_full Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_fullStr Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_full_unstemmed Epichromatin is conserved in Toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
title_sort epichromatin is conserved in toxoplasma gondii and labels the exterior parasite chromatin throughout the cell cycle
publishDate 2013
url http://sedici.unlp.edu.ar/handle/10915/125918
work_keys_str_mv AT vanagaslaura epichromatinisconservedintoxoplasmagondiiandlabelstheexteriorparasitechromatinthroughoutthecellcycle
AT dalmassomariacarolina epichromatinisconservedintoxoplasmagondiiandlabelstheexteriorparasitechromatinthroughoutthecellcycle
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AT olinsdonalde epichromatinisconservedintoxoplasmagondiiandlabelstheexteriorparasitechromatinthroughoutthecellcycle
AT angelsergioo epichromatinisconservedintoxoplasmagondiiandlabelstheexteriorparasitechromatinthroughoutthecellcycle
bdutipo_str Repositorios
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