Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?

The intracellular bacteria <i>Wolbachia pipientis</i> can manipulate host reproduction to enhance their vertical transmission. It has been reported an association between parthenogenesis and <i>Wolbachia</i> infection in weevils from the tribe Naupactini. A curing experiment...

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Autores principales: Da Cruz Cabral, Lucía Mariana, Piccinali, Romina Valeria, Lanteri, Analía Alicia, Confalonieri, Viviana A., Rodriguero, Marcela Silvina
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2020
Materias:
Biología
Wolbachia
Naupactini
Weevils
Thelytokous Parthenogenesis
Wolbachia Titers
Interacting Microbiota
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125726
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125726
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Wolbachia
Naupactini
Weevils
Thelytokous Parthenogenesis
Wolbachia Titers
Interacting Microbiota
spellingShingle Biología
Wolbachia
Naupactini
Weevils
Thelytokous Parthenogenesis
Wolbachia Titers
Interacting Microbiota
Da Cruz Cabral, Lucía Mariana
Piccinali, Romina Valeria
Lanteri, Analía Alicia
Confalonieri, Viviana A.
Rodriguero, Marcela Silvina
Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
topic_facet Biología
Wolbachia
Naupactini
Weevils
Thelytokous Parthenogenesis
Wolbachia Titers
Interacting Microbiota
description The intracellular bacteria <i>Wolbachia pipientis</i> can manipulate host reproduction to enhance their vertical transmission. It has been reported an association between parthenogenesis and <i>Wolbachia</i> infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of <i>Wolbachia</i> is required for parthenogenesis to occur. The aim of this study was to analyze <i>Wolbachia</i> infection status in the bisexual species <i>Naupactus xanthographus</i> and <i>Naupactus dissimulator</i>. <i>Wolbachia</i> infection was detected in both species from some geographic locations, not being fixed. In all positive cases, faint PCR bands were observed. Quantification through real time PCR confirmed that <i>Wolbachia</i> loads in bisexual species were significantly lower than in parthenogenetic ones; this strengthens the hypothesis of a threshold level. Strain typing showed that both species carry <i>w</i>Nau1, the most frequent in parthenogenetic Naupactini weevils. These infections seem to be recently acquired by horizontal transfer. <i>Wolbachia</i> was located throughout the whole body, which reinforce the idea of recent transmission. Moreover, we demonstrated that this strain carries the WO phage. Finally, the analysis of eubacterial 16S <i>rRNA</i> gene showed intense PCR bands for both bisexual species, suggesting –the presence of additional bacteria. Interspecific competition might explain why the parthenogenetic phenotype is not triggered.
format Articulo
Preprint
author Da Cruz Cabral, Lucía Mariana
Piccinali, Romina Valeria
Lanteri, Analía Alicia
Confalonieri, Viviana A.
Rodriguero, Marcela Silvina
author_facet Da Cruz Cabral, Lucía Mariana
Piccinali, Romina Valeria
Lanteri, Analía Alicia
Confalonieri, Viviana A.
Rodriguero, Marcela Silvina
author_sort Da Cruz Cabral, Lucía Mariana
title Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
title_short Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
title_full Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
title_fullStr Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
title_full_unstemmed Parthenogenesis in weevils of the tribe Naupactini (Coleoptera, Curculionidae) : A Wolbachia-density dependent trait?
title_sort parthenogenesis in weevils of the tribe naupactini (coleoptera, curculionidae) : a wolbachia-density dependent trait?
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/125726
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