Non-aversive photographic measurement method for subcutaneous tumours in nude mice

We have developed a new method for the measurement of subcutaneous tumour volume which consists in taking photographs of mice in their home cages, to refine the standard method of measurement with calipers. We consider this new method to be non-aversive, as it may be more compatible with mice behavi...

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Detalles Bibliográficos
Autores principales: Resasco, Agustina, Carranza Martín, Ana Cristina, Ayala, Miguel Ángel, Diaz, Silvina L., Carbone, Cecilia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2018
Materias:
Biología
Welfare
tumour model
non-aversive
mice
refinement
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125721
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125721
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Welfare
tumour model
non-aversive
mice
refinement
spellingShingle Biología
Welfare
tumour model
non-aversive
mice
refinement
Resasco, Agustina
Carranza Martín, Ana Cristina
Ayala, Miguel Ángel
Diaz, Silvina L.
Carbone, Cecilia
Non-aversive photographic measurement method for subcutaneous tumours in nude mice
topic_facet Biología
Welfare
tumour model
non-aversive
mice
refinement
description We have developed a new method for the measurement of subcutaneous tumour volume which consists in taking photographs of mice in their home cages, to refine the standard method of measurement with calipers. We consider this new method to be non-aversive, as it may be more compatible with mice behavioural preferences and, therefore, improve their welfare. Photographs are captured when mice voluntarily go into an acrylic tube containing graph paper that is later used as a scale. Tumour volumes measured with the caliper and the non-aversive photographic method were compared to those obtained by water displacement volume and weight. Behavioural and physiological changes were evaluated to assess animal welfare. Significant differences were found between measurements obtained with the caliper and the non-aversive photographic method, v. the reference volume acquired by water displacement (P < 0.001). Nevertheless, there was good consistency for these measurements when tumours were measured repeatedly, with all Intra-Class Correlation Coefficients above 0.95. Mice on which the non-aversive photographic method was employed were significantly less reluctant to establish contact with the experimenter (P < 0.001) and behaved less anxiously in a modified-Novelty Suppressed Feeding test. Particularly, statistically significant differences were found in connection with the latency to eat an almond piece (P < 0.05), the frequency of grooming (P < 0.001) and the frequency of defecation (P < 0.001). Corticosterone concentration in faeces and blood glucose were determined and no significant changes were found. Therefore, we propose the non-aversive photographic method to measure subcutaneous tumours as a way to refine methodologies in the field of experimental oncology.
format Articulo
Articulo
author Resasco, Agustina
Carranza Martín, Ana Cristina
Ayala, Miguel Ángel
Diaz, Silvina L.
Carbone, Cecilia
author_facet Resasco, Agustina
Carranza Martín, Ana Cristina
Ayala, Miguel Ángel
Diaz, Silvina L.
Carbone, Cecilia
author_sort Resasco, Agustina
title Non-aversive photographic measurement method for subcutaneous tumours in nude mice
title_short Non-aversive photographic measurement method for subcutaneous tumours in nude mice
title_full Non-aversive photographic measurement method for subcutaneous tumours in nude mice
title_fullStr Non-aversive photographic measurement method for subcutaneous tumours in nude mice
title_full_unstemmed Non-aversive photographic measurement method for subcutaneous tumours in nude mice
title_sort non-aversive photographic measurement method for subcutaneous tumours in nude mice
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/125721
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AT carranzamartinanacristina nonaversivephotographicmeasurementmethodforsubcutaneoustumoursinnudemice
AT ayalamiguelangel nonaversivephotographicmeasurementmethodforsubcutaneoustumoursinnudemice
AT diazsilvinal nonaversivephotographicmeasurementmethodforsubcutaneoustumoursinnudemice
AT carbonececilia nonaversivephotographicmeasurementmethodforsubcutaneoustumoursinnudemice
bdutipo_str Repositorios
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