Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions

Nitrated fatty acids (NO₂-FAs) are formed by the addition reaction of nitric oxide- and nitrite-derived nitrogen dioxide with unsaturated fatty acids. Nitrated fatty acids act as signaling molecules in mammals through the formation of covalent adducts with cellular thiols. The study of NO₂-FAs in pl...

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Autores principales: Di Palma, Andrés Arruebarrena, Di Fino, Luciano M., Salvatore, Sonia R., D`Ambrosio, Juan Martin, Gergoff Grozeff, Gustavo Esteban, García-Mata, Carlos, Schopfer, Francisco J., Laxalt, Ana M.
Formato: Articulo Preprint
Lenguaje:Español
Publicado: 2018
Materias:
Botánica
Nitro-oleic acid
Tomato cell suspension
ROS
Glutathione
Signalling
Plant defense
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125617
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125617
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Botánica
Nitro-oleic acid
Tomato cell suspension
ROS
Glutathione
Signalling
Plant defense
spellingShingle Botánica
Nitro-oleic acid
Tomato cell suspension
ROS
Glutathione
Signalling
Plant defense
Di Palma, Andrés Arruebarrena
Di Fino, Luciano M.
Salvatore, Sonia R.
D`Ambrosio, Juan Martin
Gergoff Grozeff, Gustavo Esteban
García-Mata, Carlos
Schopfer, Francisco J.
Laxalt, Ana M.
Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
topic_facet Botánica
Nitro-oleic acid
Tomato cell suspension
ROS
Glutathione
Signalling
Plant defense
description Nitrated fatty acids (NO₂-FAs) are formed by the addition reaction of nitric oxide- and nitrite-derived nitrogen dioxide with unsaturated fatty acids. Nitrated fatty acids act as signaling molecules in mammals through the formation of covalent adducts with cellular thiols. The study of NO₂-FAs in plant systems constitutes an interesting and emerging area. The presence of NO₂-FA has been reported in olives, peas, rice and in Arabidopsis. To gain a better understanding of the role of NO₂-FA on plant physiology, we analyzed the effects of exogenous application of nitro-oleic acid (NO₂-OA) to tomato cell cultures. We found that NO₂-OA induced reactive oxygen species (ROS) production in a dose-dependent manner via activation of NADPH oxidases, which requires calcium entry from the extracellular compartment and protein kinase activation, a mechanism that resembles the plant defense responses. NO₂-OA induced ROS production, expression of plant defense genes and led to cell death. The mechanism of action of NO₂-OA involves a reduction in the glutathione cellular pool and covalently addition reactions with protein thiols and reduced glutathione. Altogether, these results indicate that NO₂-OA triggers responses associated with plant defense, revealing its possible role as a signal molecule in biotic stress.
format Articulo
Preprint
author Di Palma, Andrés Arruebarrena
Di Fino, Luciano M.
Salvatore, Sonia R.
D`Ambrosio, Juan Martin
Gergoff Grozeff, Gustavo Esteban
García-Mata, Carlos
Schopfer, Francisco J.
Laxalt, Ana M.
author_facet Di Palma, Andrés Arruebarrena
Di Fino, Luciano M.
Salvatore, Sonia R.
D`Ambrosio, Juan Martin
Gergoff Grozeff, Gustavo Esteban
García-Mata, Carlos
Schopfer, Francisco J.
Laxalt, Ana M.
author_sort Di Palma, Andrés Arruebarrena
title Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
title_short Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
title_full Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
title_fullStr Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
title_full_unstemmed Nitro-Oleic Acid Induced Reactive Oxygen Species Formation and Plant Defense Signaling in Tomato Cell Suspensions
title_sort nitro-oleic acid induced reactive oxygen species formation and plant defense signaling in tomato cell suspensions
publishDate 2018
url http://sedici.unlp.edu.ar/handle/10915/125617
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