Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity

Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly in...

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Detalles Bibliográficos
Autores principales: Cotabarren, Juliana, Ozón, Brenda, Claver, Santiago Pablo, García Pardo, Javier, Obregón, Walter David
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Biología
Geoffroea decorticans
Antioxidant
Functional compound
Thermostable protein
Reactive oxygen species
Anticoagulant
Bioactive compound
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125447
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125447
record_format dspace
spelling I19-R120-10915-1254472023-10-23T20:46:09Z http://sedici.unlp.edu.ar/handle/10915/125447 Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity Cotabarren, Juliana Ozón, Brenda Claver, Santiago Pablo García Pardo, Javier Obregón, Walter David 2021 2021-09-23T13:30:13Z en Biología Geoffroea decorticans Antioxidant Functional compound Thermostable protein Reactive oxygen species Anticoagulant Bioactive compound Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes. Centro de Investigación de Proteínas Vegetales Articulo Articulo http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) application/pdf
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
Geoffroea decorticans
Antioxidant
Functional compound
Thermostable protein
Reactive oxygen species
Anticoagulant
Bioactive compound
spellingShingle Biología
Geoffroea decorticans
Antioxidant
Functional compound
Thermostable protein
Reactive oxygen species
Anticoagulant
Bioactive compound
Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago Pablo
García Pardo, Javier
Obregón, Walter David
Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
topic_facet Biología
Geoffroea decorticans
Antioxidant
Functional compound
Thermostable protein
Reactive oxygen species
Anticoagulant
Bioactive compound
description Geoffroea decorticans is a xerophilous deciduous tree present in most arid forests of southern South America, which is commonly used in traditional medicine. The seeds of this tree have been previously investigated for their singular chemical composition, but their protein content has been poorly investigated. Herein, we report the isolation, purification, and characterization of a set of thermostable peptides derived from Geoffroea decorticans seeds (GdAPs) with strong antioxidant and anticoagulant activities. The most potent antioxidant peptides showed a half maximal inhibitory concentration (IC50) of 35.5 ± 0.3 µg/mL determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH). They also caused a dose-dependent prolongation of the aPTT clotting time with an IC50 value of ~82 µg/mL. Interestingly, MALDI-TOF/MS analysis showed the presence of three major peptides with low molecular weights of 2257.199 Da, 2717.165 Da, and 5422.002 Da. The derived amino-acid sequence of GdAPs revealed their unique structural features, exhibiting homology with various proteins present in the genome of Arachis hypogaea. All in all, our data suggest a direct applicability of GdAPs for pharmaceutical purposes.
format Articulo
Articulo
author Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago Pablo
García Pardo, Javier
Obregón, Walter David
author_facet Cotabarren, Juliana
Ozón, Brenda
Claver, Santiago Pablo
García Pardo, Javier
Obregón, Walter David
author_sort Cotabarren, Juliana
title Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
title_short Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
title_full Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
title_fullStr Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
title_full_unstemmed Purification and Identification of Novel Antioxidant Peptides Isolated from Geoffroea decorticans Seeds with Anticoagulant Activity
title_sort purification and identification of novel antioxidant peptides isolated from geoffroea decorticans seeds with anticoagulant activity
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/125447
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