Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus

It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation wer...

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Autores principales: Scarlet, Dragos, Handschuh, Stephan, Reichart, Ursula, Podico, Giorgia, Ellerbrock, Robyn E., Demyda Peyrás, Sebastián, Canisso, Igor F., Walter, Ingrid, Aurich, Christine
Formato: Articulo
Lenguaje:Inglés
Publicado: 2021
Materias:
Ciencias Veterinarias
Veterinaria
gonad
germ cell
genital duct
fetus
horse
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125446
https://www.mdpi.com/2076-2615/11/8/2422
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125446
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Veterinaria
gonad
germ cell
genital duct
fetus
horse
spellingShingle Ciencias Veterinarias
Veterinaria
gonad
germ cell
genital duct
fetus
horse
Scarlet, Dragos
Handschuh, Stephan
Reichart, Ursula
Podico, Giorgia
Ellerbrock, Robyn E.
Demyda Peyrás, Sebastián
Canisso, Igor F.
Walter, Ingrid
Aurich, Christine
Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
topic_facet Ciencias Veterinarias
Veterinaria
gonad
germ cell
genital duct
fetus
horse
description It was the aim of this study to characterize the development of the gonads and genital ducts in the equine fetus around the time of sexual differentiation. This included the identification and localization of the primordial germ cell population. Equine fetuses between 45 and 60 days of gestation were evaluated using a combination of micro-computed tomography scanning, immunohistochemistry, and multiplex immunofluorescence. Fetal gonads increased in size 23-fold from 45 to 60 days of gestation, and an even greater increase was observed in the metanephros volume. Signs of mesonephros atrophy were detected during this time. Tubular structures of the fetal testes were present from day 50 onwards, whereas cell clusters dominated in the fetal ovary. The genital ducts were well-differentiated and presented a lumen in all samples. No sign of mesonephric or paramesonephric duct degeneration was detected. Expression of AMH was strong in the fetal testes but absent in ovaries. Irrespective of sex, primordial germ cells selectively expressed LIN28. Migration of primordial germ cells from the mesonephros to the gonad was detected at 45 days, but not at 60 days of development. Their number and distribution within the gonad were influenced (p < 0.05) by fetal sex. Most primordial germ cells (86.8 ± 3.2% in females and 84.6 ± 4.7% in males) were characterized as pluripotent according to co-localization with CD117. However, only a very small percentage of primordial germ cells were proliferating (7.5 ± 1.7% in females and 3.2 ± 1.2% in males) based on co-localization with Ki67. It can be concluded that gonadal sexual differentiation in the horse occurs asynchronously with regard to sex but already before 45 days of gestation.
format Articulo
Articulo
author Scarlet, Dragos
Handschuh, Stephan
Reichart, Ursula
Podico, Giorgia
Ellerbrock, Robyn E.
Demyda Peyrás, Sebastián
Canisso, Igor F.
Walter, Ingrid
Aurich, Christine
author_facet Scarlet, Dragos
Handschuh, Stephan
Reichart, Ursula
Podico, Giorgia
Ellerbrock, Robyn E.
Demyda Peyrás, Sebastián
Canisso, Igor F.
Walter, Ingrid
Aurich, Christine
author_sort Scarlet, Dragos
title Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
title_short Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
title_full Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
title_fullStr Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
title_full_unstemmed Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus
title_sort sexual differentiation and primordial germ cell distribution in the early horse fetus
publishDate 2021
url http://sedici.unlp.edu.ar/handle/10915/125446
https://www.mdpi.com/2076-2615/11/8/2422
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