Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus

The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the...

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Detalles Bibliográficos
Autores principales: Pascual, Agustina, Vilardo, Emiliano Salvador, Taibo, Catalina, Sabio y García, J., Rivera Pomar, Rolando Víctor
Formato: Articulo Preprint
Lenguaje:Inglés
Publicado: 2020
Materias:
Ciencias Exactas
Bioquímica
oogenesis
Rhodnius prolixus
Bicaudal C
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/125401
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-125401
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bioquímica
oogenesis
Rhodnius prolixus
Bicaudal C
spellingShingle Ciencias Exactas
Bioquímica
oogenesis
Rhodnius prolixus
Bicaudal C
Pascual, Agustina
Vilardo, Emiliano Salvador
Taibo, Catalina
Sabio y García, J.
Rivera Pomar, Rolando Víctor
Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
topic_facet Ciencias Exactas
Bioquímica
oogenesis
Rhodnius prolixus
Bicaudal C
description The morphology and physiology of the oogenesis have been well studied in the vector of Chagas disease Rhodnius prolixus. However, the molecular interactions that regulate the process of egg formation, key for the reproductive cycle of the vector, is still largely unknown. In order to understand the molecular and cellular basis of the oogenesis we examined the function of the gene Bicaudal C (BicC) during oogenesis and early development of R. prolixus. We show that R. prolixus BicC (Rp-BicC) gene is expressed in the germarium, with cytoplasmic distribution, as well as in the follicular epithelium of the developing oocytes. RNAi silencing of Rp-BicC resulted in sterile females that lay few, small, non-viable eggs. The ovaries are reduced in size and show a disarray of the follicular epithelium. This indicates that Rp-BicC has a central role in the regulation of oogenesis. Although the follicular cells are able to form the chorion, the uptake of vitelline by the oocytes is compromised. We show evidence that the polarity of the follicular epithelium and the endocytic pathway, which is crucial for the proper yolk deposition, are affected. This study provides insights into the molecular mechanisms underlying oocyte development and show that Rp-BicC is important for de developenta of the egg and, therefore, a key player in the reproduction of this Chagas disease vector.
format Articulo
Preprint
author Pascual, Agustina
Vilardo, Emiliano Salvador
Taibo, Catalina
Sabio y García, J.
Rivera Pomar, Rolando Víctor
author_facet Pascual, Agustina
Vilardo, Emiliano Salvador
Taibo, Catalina
Sabio y García, J.
Rivera Pomar, Rolando Víctor
author_sort Pascual, Agustina
title Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_short Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_full Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_fullStr Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_full_unstemmed Bicaudal C is required for the function of the follicular epithelium during oogenesis in Rhodnius prolixus
title_sort bicaudal c is required for the function of the follicular epithelium during oogenesis in rhodnius prolixus
publishDate 2020
url http://sedici.unlp.edu.ar/handle/10915/125401
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