A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern

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Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We fi...

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Autores principales:	Muñoz Nava, Luis Manuel, Álvarez, Hugo Ariel, Flores Flores, Marycruz, Chara, Osvaldo, Nahmad, Marcos
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2020
Materias:	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/125216
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-125216
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
spellingShingle	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
topic_facet	Biología Organ growth Patterning Vestigial Cell recruitment Scaling Drosophila wing disc
description	Organs mainly attain their size by cell growth and proliferation, but sometimes also grow through recruitment of undifferentiated cells. Here we investigate the participation of cell recruitment in establishing the pattern of Vestigial (Vg), the product of the wing selector gene in Drosophila. We find that the Vg pattern overscales along the dorsal-ventral (DV) axis of the wing imaginal disc, i.e., it expands faster than the DV length of the pouch. The overscaling of the Vg pattern cannot be explained by differential proliferation, apoptosis, or oriented-cell divisions, but can be recapitulated by a mathematical model that explicitly considers cell recruitment. When impairing cell recruitment genetically, we find that the Vg pattern almost perfectly scales and adult wings are approximately 20% smaller. Conversely, impairing cell proliferation results in very small wings, suggesting that cell recruitment and cell proliferation additively contribute to organ growth in this system. Furthermore, using fluorescent reporter tools, we provide direct evidence that cell recruitment is initiated between early and mid third-instar larval development. Altogether, our work quantitatively shows when, how, and by how much cell recruitment shapes the Vg pattern and drives growth of the Drosophila wing.
format	Articulo Articulo
author	Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos
author_facet	Muñoz Nava, Luis Manuel Álvarez, Hugo Ariel Flores Flores, Marycruz Chara, Osvaldo Nahmad, Marcos
author_sort	Muñoz Nava, Luis Manuel
title	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_short	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_full	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_fullStr	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_full_unstemmed	A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern
title_sort	dynamic cell recruitment process drives growth of the drosophila wing by overscaling the vestigial expression pattern
publishDate	2020
url	http://sedici.unlp.edu.ar/handle/10915/125216
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bdutipo_str	Repositorios
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A dynamic cell recruitment process drives growth of the Drosophila wing by overscaling the vestigial expression pattern

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